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Tm (cr)Tj 9.5 0 0 9.5 283.8047 458.5889 Tm (\),)Tj -25.1 -1.2055 TD [(lysine)-503.9(propionylation)-510(\(K)]TJ 7.1249 0 0 6.3326 142.8661 445.5495 Tm (pr)Tj 9.5 0 0 9.5 149.159 447.1369 Tm [(\),)-507.2(lysine)-503.9(glutarylation)-506.8(\(K)]TJ 7.1249 0 0 6.3326 248.485 445.5495 Tm (glu)Tj 9.5 0 0 9.5 257.2157 447.1369 Tm [(\),)-507.2(lysine)]TJ -22.3011 -1.2114 TD 0.0364 Tc [(butyryl)5.9(a)2(tion)-532.6(\(K)]TJ 7.1249 0 0 6.3326 111.1748 434.0408 Tm 0.049 Tc (bu)Tj 9.5 0 0 9.5 119.1118 435.6282 Tm 0.0364 Tc [(\),)-533.3(ly)5.1(sine)-532.3(malo)5.3(nyl)5.9(a)-4(tio)5.3(n)-538.5(\(K)]TJ 7.1249 0 0 6.3326 228.6992 434.0408 Tm 0.0474 Tc [(ma)7(l)]TJ 9.5 0 0 9.5 240.3779 435.6282 Tm 0.0355 Tc [(\),)-534.2(lysine)]TJ /F14 1 Tf 4.2012 0 TD 0 Tc (b)Tj /F11 1 Tf 0.5789 0 TD (-)Tj -25.3088 -1.2114 TD [(hy)16.3(dr)12.7(ox)19.1(yb)15.1(ut)16.4(yr)16.4(yl)15.7(at)16.9(io)14(n)-312.4(\()0(K)]TJ 7.1249 0 0 6.3326 134.1921 422.5322 Tm [(bh)20.5(b)]TJ 9.5 0 0 9.5 144.8504 424.1196 Tm [(\),)-304.3(an)11.2(d)-309.5(l)0(y)15.8(s)0(i)15.3(n)0(e)-308.1(2)13.5(-h)13.7(yd)17.2(ro)16.6(xy)12.9(is)15.3(ob)15.3(u)12.8(t)0(y)14(r)0(y)16.4(l)0(a)18.6(t)0(i)13(o)0(n)]TJ -10.4732 -1.2114 TD (\(K)Tj 7.1249 0 0 6.3326 54.7654 411.0235 Tm [(hi)19.2(b)]TJ 9.5 0 0 9.5 63.7795 412.6109 Tm [(\))-367.2(a)0(r)19.2(e)-371.7(vi)13.8(ta)16.9(l)-364.7(f)0(o)15.2(r)-370(ef)]TJ /F12 1 Tf 6.457 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ci)19.6(en)14.1(t)-366.4(r)0(e)16.1(g)0(u)11.2(l)0(a)18.6(t)0(i)13(o)0(n)-355.4(o)0(f)-360.8(m)0(a)13.7(n)0(y)-361.6(p)0(r)18.7(o)0(k)16.3(a)0(r)13.3(y)0(o)15.1(t)0(i)18.9(c)-371.7(a)13.3(n)0(d)]TJ -8.9216 -1.2114 TD [(eu)17(ka)12.9(r)11.9(y)0(o)15.1(t)0(i)13(c)-288.2(pr)12.7(ot)14.3(ei)19.6(ns)-282.6(\()]TJ 0 0 0 0.7 k 8.2234 0 TD [(Ya)17.7(ng)-284.1(et)-284.6(al)18.6(.,)-286.1(20)15.1(15)]TJ 0 0 0 1 k 6.6002 0 TD (\).)Tj -13.5585 -1.2114 TD [(Protein)-438.7(lysine)-438.2(succinylation)-440.1(\(K)]TJ 7.1249 0 0 6.3326 179.2629 388.0062 Tm (suc)Tj 9.5 0 0 9.5 188.5039 389.5936 Tm [(\),)-441.6(also)-436.2(referred)-441.5(to)-439.3(as)-434.3(N)]TJ /F14 1 Tf 9.7989 0 TD (e)Tj /F11 1 Tf 0.4416 0 TD (-)Tj -25.3088 -1.2114 TD [(succinylation,)-287.8(is)-283.1(a)-285.1(newly)-283.8(identi)]TJ /F12 1 Tf 12.3232 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(ed)-287.4(and)-280.4(evolutionarily)-290.6(conserved)]TJ -12.8543 -1.2055 TD [(reversible)-315.7(PTM)-308.2(from)-312.7(prokaryotes)-310.8(to)-314(eukaryotes.)-314(It)-307.9(transfers)-316.6(the)]TJ 0 -1.2114 TD [(succinyl)-307.9(group)-305.6(\(-CO-CH)]TJ 7.1249 0 0 6.3326 140.9386 353.5369 Tm (2)Tj 9.5 0 0 9.5 144.3968 355.1243 Tm (-CH)Tj 7.1249 0 0 6.3326 161.1779 353.5369 Tm (2)Tj 9.5 0 0 9.5 164.6362 355.1243 Tm [(-CO-\))-305.1(from)-300.7(the)-302.6(succinyl-CoA)-312.9(to)]TJ -12.5559 -1.2114 TD 0.0217 Tc [(the)-529.1(l)3.2(y)-3.7(s)-2.2(in)-4.2(e)-523.1(r)-2.2(e)-3.9(s)-2.2(idu)-7.3(e)-523.1(of)-528.7(the)-523.1(p)-1.4(rote)-3.9(i)-4.7(n)-523.4(moi)-4.7(e)-3.9(ty)-3.7(,)-524.2(r)-2.2(esult)-4.5(i)1.2(n)-4.2(g)-522.9(i)-4.7(n)-523.4(the)]TJ T* 0.0108 Tc [(formation)-510.4(o)-2.4(f)-509.8(s)-1.2(uccinyl)4.2(-)0.8(lysine)-516.1(\()]TJ 0 0 0 0.7 k 12.8364 0 TD [(Zhang)-509.8(e)3.2(t)-510.6(a)0.3(l.,)-511.1(2011)]TJ 0 0 0 1 k 8.0086 0 TD 0.0119 Tc [(\).)-510.1(Recently)4.4(,)]TJ -20.845 -1.2114 TD 0 Tc [(numerous)-420.9(lysine-succinylated)-429.8(proteins)-421(have)-419.7(been)-418.6(identi)]TJ /F12 1 Tf 22.9755 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(ed)-418.7(in)]TJ -23.5066 -1.2114 TD [(various)-247.9(bacterial)-240.4(pathogens,)-248.4(such)-245.7(as)]TJ /F15 1 Tf 14.4298 0 TD [(Mycobacterium)-244.4(tuberculosis)]TJ /F11 1 Tf 11.0103 0 TD (,)Tj /F15 1 Tf -25.4401 -1.2055 TD [(Porphyromonas)-368.2(gingivalis)]TJ /F11 1 Tf 10.2882 0 TD (,)Tj /F15 1 Tf 0.5789 0 TD [(Candida)-361.6(albicans)]TJ /F11 1 Tf 7.3283 0 TD (\()Tj 0 0 0 0.7 k 0.3461 0 TD [(Yang)-361.9(et)-356.2(al.,)-362.9(2015)]TJ 0 0 0 1 k 6.8986 0 TD (;)Tj 0 0 0 0.7 k -25.4401 -1.2114 TD [(Zheng)-212.1(et)-213(al.,)-213.8(2016)]TJ 0 0 0 1 k 6.9344 0 TD (;)Tj 0 0 0 0.7 k 0.4297 0 TD [(Wu)-212.8(et)-207(al.,)-213.8(2019)]TJ 0 0 0 1 k 5.8781 0 TD [(\),)-214.8(and)-208.8(so)-210.2(on.)-215(Many)-209.3(are)-215.2(enzymes)]TJ -13.2422 -1.2114 TD [(involved)-237.3(in)-237.4(various)-236(metabolic)-236.6(pathways)-236.5(and)-232.6(regulation)-236.8(of)-235.4(several)]TJ T* [(central)-436.9(metabolic)-439.5(processes)-441.4(in)-434.3(the)-439.9(bacteria)-436.7(such)-436.7(as)-440.3(glycolysis,)]TJ T* [(gluconeogenesis,)-415.8(the)-404(tricarboxylic)-417.7(acid)-407.9(cycle)-407.2(\(TCA)-409.3(cycle\),)-413.1(and)]TJ T* 0.0134 Tc [(fatty)-513.3(acid)-510.9(metabo)6.1(lism)-517.3(\()]TJ 0 0 0 0.7 k 10.2166 0 TD 0.0158 Tc [(Xi)7.3(e)-511.1(e)2.1(t)-511.7(a)5.2(l.,)-512.2(2)5.5(01)5.5(4)]TJ 0 0 0 1 k 6.7852 0 TD 0.0129 Tc [(\).)-515.1(Fu)-4.2(rthermore,)-515.1(N)]TJ /F14 1 Tf 7.8534 0 TD 0 Tc (e)Tj /F11 1 Tf 0.4535 0 TD (-)Tj -25.3088 -1.2055 TD [(su)-17.1(c)-7.7(c)0(i)-10.3(n)-8(y)-7.5(l)-6.6(at)-12.9(i)-8.5(o)-7.2(n)-515.3(h)0(a)-10.7(s)-513.2(b)-7.2(e)-7.7(e)-7.7(n)-515.3(re)-13.7(po)-12.4(rt)-14.3(e)-7.7(d)-512.4(i)-8.5(n)-515.3(ma)-16.1(n)-8(y)-508.8(p)-11.1(r)0(o)-13.2(t)-8.3(e)-7.7(i)0(n)-517.9(s)0(u)-17.1(b)-7.2(st)-8.3(r)-12(a)0(t)-12.9(e)-7.7(s)]TJ 0 -1.2114 TD 0.0193 Tc [(and)-522.9(i)-1.2(nvo)-5.9(l)0.8(ved)-522.9(i)-1.2(n)-525.8(t)-1(he)-525.5(re)-6.3(gulation)-525.8(of)-519.2(c)-6.3(e)-0.3(ll)-5.2(ul)-5.2(ar)-523.8(physiol)-5.2(o)0.1(gy)-525.3(an)-6.6(d)]TJ T* 0 Tc [(metabolism)-449.4(in)-452.2(both)-446.7(prokaryotic)-450.6(and)-447.5(eukaryotic)-451(cells)-452.4(\()]TJ 0 0 0 0.7 k 22.7427 0 TD (Nadine)Tj -22.7427 -1.2114 TD [(et)-386(al.,)-380.9(2017)]TJ 0 0 0 1 k 4.5235 0 TD [(\).)-387.9(This)-385.3(PTM)-379.8(can)-384.4(make)-388.2(prominent)-386.9(modi)]TJ /F12 1 Tf 16.5961 0 TD ()Tj /F11 1 Tf 0.5371 0 TD [(cations)-384.6(in)]TJ -21.6566 -1.2114 TD [(structure)-346.8(regulation)-344.2(and)-346(protein)-343.4(function.)-343.7(The)-342.7(identi)]TJ /F12 1 Tf 21.5373 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(cation)-342.8(of)]TJ -22.0684 -1.2114 TD 0.0134 Tc [(protei)4.9(n)-513.8(s)1.4(ucciny)5.9(lat)5.1(i)-1.1(on)-507.8(si)4.9(tes)-511.8(h)1.3(as)-511.8(important)-508.1(i)-1.1(mpl)6.8(i)-1.1(catio)6.2(n)-0.6(s)-511.8(w)-1.8(i)4.9(t)-0.9(h)]TJ T* 0 Tc [(regards)-353.3(to)-355.7(understanding)-356.8(of)-354.8(cellular)-355.4(physiology)-355.8(and)-352(pathology,)]TJ 0 -1.2055 TD [(potentially)-215.9(leading)-215.2(to)-206.5(valuable)-217.1(information)-210.8(for)-211.6(drug)-214.8(development)]TJ 0 -1.2114 TD 0.0178 Tc [(an)3.8(d)-512.5(b)-1.4(i)3.3(o)4.6(me)4.1(di)3.3(cal)-514(r)5.8(es)5.8(ear)5.8(c)-1.8(h)5.7(.)-516.1(I)4.7(n)-515.4(re)4.1(c)4.1(e)-1.8(n)3.8(t)-515.7(t)3.5(i)3.3(me)4.1(s,)-510.2(hi)3.3(gh)5.7(-t)3.5(hr)5.8(oug)4.4(h)-0.2(p)6.7(u)0.7(t)]TJ T* 0 Tc [(approaches)-412.4(in)-410.4(conjunction)-410.9(with)-408.2(mass)-410.1(spectrometry)-411.4(have)-407.8(been)]TJ T* [(widely)-307.4(applied)-305.5(to)-302(identify)-305.8(the)-302.6(K)]TJ 7.1249 0 0 6.3326 166.9039 112.0252 Tm (suc)Tj 9.5 0 0 9.5 179.0929 113.6126 Tm [(in)-303(several)-302.7(organisms)-310.1(ranging)]TJ -14.0777 -1.2114 TD [(from)-336.5(bacteria)-335.2(to)-331.9(humans)-333.8(\()]TJ 0 0 0 0.7 k 10.7895 0 TD [(Colak)-335.6(et)-332.3(al.,)-333.1(2013)]TJ 0 0 0 1 k 7.0955 0 TD (;)Tj 0 0 0 0.7 k 0.549 0 TD [(Li)-330.9(et)-338.3(al.,)-333.1(2014)]TJ 0 0 0 1 k 5.5499 0 TD (;)Tj 0 0 0 0.7 k 0.555 0 TD (Jin)Tj -24.539 -1.2114 TD [(and)-238.6(Wu,)-239.5(2016)]TJ 0 0 0 1 k 5.5977 0 TD (;)Tj 0 0 0 0.7 k 0.4535 0 TD [(Xu)-237.4(et)-236.8(al.,)-237.6(2016)]TJ 0 0 0 1 k 5.6394 0 TD (;)Tj 0 0 0 0.7 k 0.4535 0 TD [(Feng)-237.2(et)-236.8(al.,)-237.6(2017)]TJ 0 0 0 1 k 6.4033 0 TD (;)Tj 0 0 0 0.7 k 0.4595 0 TD [(Song)-235.1(et)-236.8(al.,)-237.6(2017)]TJ 0 0 0 1 k 6.4331 0 TD (;)Tj 0 0 0 0.7 k -25.4401 -1.2055 TD [(Xie)-302.2(et)-302.5(al.,)-297.3(2017)]TJ 0 0 0 1 k 5.9856 0 TD (;)Tj 0 0 0 0.7 k 0.5192 0 TD [(Zhang)-298.5(et)-302.5(al.,)-303.3(2017)]TJ 0 0 0 1 k 7.2209 0 TD (\).)Tj /F15 1 Tf 14.9908 66.4558 TD [(Vibrio)-323.6(alginolyticus)]TJ /F11 1 Tf 8.0742 0 TD [(is)-324.9(a)-326.9(Gram-negative)-332.9(halophilic)-323.8(bacterium)]TJ -9.3394 -1.2114 TD [(and)-238.6(an)-233.4(etiological)-241.8(agent)-239(of)-235.4(vibriosis,)-241.5(mainly)-237.8(found)-235.9(in)-237.4(marine)-243.4(and)]TJ T* 0.0132 Tc [(estu)-3.9(arine)-513.7(e)-0.5(nvironments)-4.7(.)-508.8(O)-5.7(u)2.1(tbreaks)-512(c)-0.5(ause)-513.7(h)-4.8(i)4.7(gh)-518.1(mortality)-513.5(i)-1.3(n)]TJ T* 0 Tc [(marine)-338.8(animals)-342.4(with)-342.6(serious)-339.1(economic)-346.2(losses)-337.9(worldwide.)-342.3(Being)]TJ T* [(a)-505.9(z)-7.7(oon)-10.6(oti)-12.2(c)-509(pat)-12.1(ho)-7.4(ge)-9.2(n,)-512.1(it)-506.2(n)-8(o)0(t)-510.9(o)0(n)-9.3(l)0(y)-509.4(cau)-11.5(se)-7.7(s)-507.3(v)-7.5(ib)-9.8(ri)-8.6(os)-7.3(is)-509.9(in)-511.9(ma)-10.2(ri)-8.6(ne)]TJ T* 0.0349 Tc [(animals,)-534.8(but)-534.4(a)0.5(lso)-533.3(c)-2.6(auses)-538(f)3.6(oodborne)-533.8(r)-0.9(elated)-531.2(infections)-538(in)]TJ 0 -1.2055 TD 0 Tc [(humans)-321.9(by)-325.1(consumption)-321.7(of)-325(contaminated)-322.6(raw)-324.2(and)-322.1(half-cooked)]TJ 0 -1.2114 TD [(seafood)-290(\()]TJ 0 0 0 0.7 k 3.6641 0 TD [(Dan)-292.2(et)-290.5(al.,)-291.3(2019)]TJ 0 0 0 1 k 6.3377 0 TD (;)Tj 0 0 0 0.7 k 0.5132 0 TD [(Zheng)-289.7(et)-290.5(al.,)-291.3(2019)]TJ 0 0 0 1 k 7.1672 0 TD [(\).)-292.4(Moreover,)-295.1(several)]TJ -17.6822 -1.2114 TD [(researchers)-280.4(frequently)-276.5(reported)-280.1(antibiotic)-274.1(resistant)-278.4(strains)-274.3(of)-277.2(the)]TJ T* [(bacterium)-413(from)-414.1(aquaculture)-416.3(and)-411.7(clinical)-416.4(settings)-412.5(\()]TJ 0 0 0 0.7 k 20.851 0 TD [(Horii)-410.2(et)-409.9(al.,)]TJ -20.851 -1.2114 TD (2005)Tj 0 0 0 1 k 1.9156 0 TD (;)Tj 0 0 0 0.7 k 0.555 0 TD [(Ferrini)-336.4(et)-332.3(al.,)-333.1(2008)]TJ 0 0 0 1 k 7.4834 0 TD (;)Tj 0 0 0 0.7 k 0.555 0 TD [(Xiong)-335.2(et)-332.3(al.,)-333.1(2010)]TJ 0 0 0 1 k 7.1612 0 TD (\).)Tj /F15 1 Tf 0.8951 0 TD [(V.)-334.3(alginolyticus)]TJ /F11 1 Tf 6.4689 0 TD (is)Tj -25.0343 -1.2114 TD [(able)-229.1(to)-224.4(form)-223.1(bio)]TJ /F12 1 Tf 6.3138 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(lms)-227.1(and)-226.7(is)-223.4(capable)-228.6(of)]TJ /F12 1 Tf 8.3846 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(agellar)-228.6(mediated)-231.8(motility)]TJ -15.7606 -1.2055 TD (\()Tj 0 0 0 0.7 k 0.3461 0 TD [(Echazarreta)-330.9(and)-334.1(Klose,)-331.1(2019)]TJ 0 0 0 1 k 11.5056 0 TD [(\).)-328.2(It)-331.8(also)-328.8(secretes)-331.6(several)-332.6(virulence)]TJ -11.8518 -1.2114 TD [(factors)-399.4(such)-394.9(as,)-395.3(serine)-402(protease,)-395.7(hemolysin,)-400.3(exopolysaccharide,)]TJ T* [(siderophores,)-308.4(and)-310.2(cell)-303.1(surface)-313.1(hydrophobicity)-307.2(products)-307.4(through)]TJ T* [(v)-7.5(a)-10.6(ri)-14.5(o)-13.2(u)0(s)-524.4(m)-11.5(e)-7.7(t)-8.3(a)-10.6(b)-7.3(o)-7.3(l)-6.6(i)-8.5(c)-520.9(pa)-15.7(t)-8.3(h)-12.1(w)-9.3(a)0(y)-12.1(s)-519.2(\()]TJ 0 0 0 0.7 k 12.6097 0 TD 0.0088 Tc [(Yang)-511.9(et)-506.8(al.,)-513.2(2)8.8(0)-5.9(15)]TJ 0 0 0 1 k 7.4655 0 TD 0 Tc (;)Tj 0 0 0 0.7 k 0.74 0 TD [(He)-17.5(rn)-14(a)]TJ /F16 1 Tf 2.5005 0.0418 TD ()Tj /F11 1 Tf 0.0597 -0.0418 TD 0.0084 Tc (ndez-)Tj -23.3753 -1.2114 TD 0 Tc [(Robles)-489(et)-493.4(al.,)-488.3(2016)]TJ 0 0 0 1 k 7.8713 0 TD (;)Tj 0 0 0 0.7 k 0.7102 0 TD [(Santhakumari)-495(et)-487.5(al.,)-494.2(2017)]TJ 0 0 0 1 k 10.8313 0 TD (;)Tj 0 0 0 0.7 k 0.7102 0 TD [(Huang)-488.6(et)-487.5(al.,)]TJ -20.1229 -1.2114 TD (2018)Tj 0 0 0 1 k 1.9156 0 TD [(\),)-274.5(which)-270.5(all)-274.4(contribute)-277.2(to)-272.2(mechanisms)-274.7(of)-271.2(pathogenicity)-274.3(that)]TJ -1.9156 -1.2055 TD [(require)-300.9(further)-309.2(understanding.)]TJ 1.2651 -1.2114 TD 0.0136 Tc [(Comprehensive)-513.3(l)1(ysine)-513.3(s)1.6(uccinylome)-513.3(studies)-511.6(c)-0.1(onducted)-510.7(in)]TJ -1.2651 -1.2114 TD 0 Tc [(dif)62.5(ferent)-450.9(bacterial)-449.3(pathogens)-448.5(have)-449.6(revealed)-446.8(the)-445.8(importance)-450.8(of)]TJ T* [(th)15.4(is)-211.5(PT)12.3(M.)-215.9(Ho)18.7(we)18.8(ve)14.7(r,)-217.7(to)-212.5(th)15.4(e)-222.5(b)0(e)14.9(s)11.9(t)-223.2(o)0(f)-217.5(o)0(u)17.4(r)-220.8(kn)15.5(ow)13.3(le)21.5(dg)11.2(e,)-213.4(no)-212.2(su)12.7(cc)14.4(in)19.2(yl)15.8(at)16.9(ed)]TJ T* 0.0269 Tc [(prot)-5.3(ei)-5.5(ns)-540.1(have)-541.8(be)-4.7(en)-542.1(disco)-4.2(v)-4.4(ered)-539.2(so)-541.3(far)-540.1(i)0.4(n)]TJ /F15 1 Tf 18.6788 0 TD 0.0278 Tc [(V.)-536.9(alg)-3.8(i)-0.6(no)-5.5(lyticus)]TJ /F11 1 Tf 6.7614 0 TD 0 Tc (,)Tj -25.4401 -1.2114 TD 0.0187 Tc [(wh)-5.3(ich)-530.4(p)1.6(resents)-524.4(a)-529(foremost)-526.7(obstacle)-526.1(f)-0.6(or)-524.4(u)-4.3(n)4.7(d)-4.4(e)-0.9(rstanding)-525.9(the)]TJ T* 0.0195 Tc [(re)-6.1(gu)-3.6(latory)-531(m)-4(echanis)-4.4(m)-529.1(o)0.3(f)-530.9(K)]TJ 7.1249 0 0 6.3326 421.6251 432.907 Tm 0.0275 Tc (suc)Tj 9.5 0 0 9.5 436.5353 434.4377 Tm 0.0206 Tc [(in)-524.5(this)-528.5(pathogen)-5.3(.)-525.3(W)-2(e)-530.2(h)2.6(ave)]TJ -13.7256 -1.2055 TD 0 Tc [(th)15.4(er)16.1(ef)14.8(or)16.6(e)-389.6(c)0(o)14.9(n)0(d)16.7(u)0(c)17(t)0(e)13.8(d)-393.1(th)15.4(e)]TJ /F12 1 Tf 10.0316 0 TD ()Tj /F11 1 Tf 0.5252 0 TD -0.0152 Tc [(rs)-15.2(t)-399.5(s)-15.2(ys)-15.2(te)-15.2(ma)-15.2(ti)-15.2(c)-401.4(a)-15.2(na)-15.2(ly)-15.2(si)-15.2(s)-399.7(t)-15.2(o)-394.8(i)-15.2(de)-15.2(nt)-15.2(if)-15.2(y)-394.1(t)-15.2(he)]TJ -10.5568 -1.2114 TD 0 Tc [(ta)16.9(rg)16.4(et)13.8(s)-370(o)0(f)-360.8(t)0(h)15.4(i)0(s)-360.7(K)]TJ 7.1249 0 0 6.3326 368.6739 409.8897 Tm [(su)24.7(c)]TJ 9.5 0 0 9.5 381.3165 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[(pH)-390.9(8.5\))-390.7(and)-387.8(8)-386.3(M)-392.1(urea.)-390.3(Protein)-390.9(concentration)-395.9(was)-389.9(determined)]TJ T* [(with)-300.8(a)-303(Bradford)-300(assay)-303.2(\()]TJ 0 0 0 0.7 k 9.3991 0 TD [(He)-298(et)-302.5(al.,)-303.3(2016)]TJ 0 0 0 1 k 5.8364 0 TD (\).)Tj /F1 1 Tf 11.9999 0 0 11.9999 45.3543 584.4471 Tm [(Enrichment)-305.6(of)-302.4(Lysine-Succinylated)]TJ 0 -1.0394 TD (Peptides)Tj /F11 1 Tf 9.5 0 0 9.5 45.3543 560.466 Tm [(Approximately)-295.9(10)-295.2(mg)-293.5(of)-295.1(protein)-289.7(sample)-298.2(was)-294.4(used)-292.6(for)-295.1(reduction)]TJ 0 -1.2114 TD [(and)-435.5(alkylation)-432.4(with)-432.1(10)-432.5(mM)-433.5(DTT)-436(and)-435.5(20)-432.5(mM)-433.5(iodoacetamide)]TJ T* [(\(IAA\),)-408.6(respectively,)-417.4(as)-404.5(described)-413.3(previously)-413.5(\()]TJ 0 0 0 0.7 k 18.5534 0 TD [(Yao)-407.3(et)-409.9(al.,)-410.7(2019)]TJ 0 0 0 1 k 6.5465 0 TD (\).)Tj -25.1 -1.2114 TD [(The)-235.3(treated)-232.8(sample)-232.6(was)-234.7(digested)-235.1(to)-236.4(peptides)-232.8(using)-238.1(trypsin)-234.5(at)-233.7(1:20)]TJ 0 -1.2055 TD [(ratio)-392.8(\(m/v\))-395.5(at)-394.9(37C)-392.3(for)-390.6(16)-390.7(h.)-396.8(The)-390.4(lysine-succinylated)-400(peptides)]TJ 0 -1.2114 TD [(were)-239.6(enriched)-236(by)-235.5(immunoaf)]TJ /F12 1 Tf 11.1237 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(nity)-235.3(using)-238.1(agarose-conjugated)-241.1(anti-)]TJ -11.6548 -1.2114 TD [(succinyllysine)-209(antibody)-205.8(\(PTM)-203.9(Biolabs)-203.2(Inc.,)-201.6(Hangzhou,)-208.2(China\),)-206.7(as)]TJ T* [(previously)-467.2(described)-467(\()]TJ 0 0 0 0.7 k 9.1604 0 TD [(Yang)-463.3(et)-463.6(al.,)-464.4(2015)]TJ 0 0 0 1 k 7.2089 0 TD [(\).)-465.4(Brie)]TJ /F12 1 Tf 2.6496 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(y,)-463.8(the)-463.7(digested)]TJ -19.55 -1.2114 TD [(peptides)-364.1(were)-358.9(incubated)-360.9(with)-354.5(anti-succinyllysine)-367.9(agarose)-359.9(beads)]TJ T* [(overnight)-317.5(at)-311.3(4C)-316.3(in)-315(NETN)-311.9(buf)57.8(fer)-313.5(\(100)-314.7(mM)-314.1(NaCl,)-313.8(50)-313.1(mM)-314.1(Tris-)]TJ T* [(HCl,)-318.1(1)-314.7(m)0(M)-320.1(EDTA,)-317.1(and)-316.2(0.5%)-317.8(\(v/v\))-319.8(Nonidet)-314.5(P-40,)-318.9(pH)-319.3(8.0\).)-315.9(The)]TJ 0 -1.2055 TD [(enriched)-236(peptides)-232.8(were)-227.6(eluted)-231.6(with)-229.2(1%)-227.1(tri)]TJ /F12 1 Tf 16.3932 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(uoroacetic)-231.4(acid)-228.9(\(TFA\))]TJ -16.9243 -1.2114 TD [(an)-12.6(d)-512.4(d)0(e)-12.9(s)0(a)-10.6(l)-6.6(te)-10.1(d)-512.4(w)0(i)-11.9(t)0(h)-515.7(C)-6.4(18)-516(Zi)-12.3(pT)-11.8(i)-8.5(p)0(s)-518.4(\()0(M)-7.3(i)-8.5(l)-6.6(l)0(i)-9.2(p)0(o)-12.4(r)0(e)-13.7(,)-510.1(B)-7.8(u)0(r)-11.2(l)-6.6(in)-10.6(g)-7.5(t)0(o)-9.6(n)0(,)-518.1(M)0(A)-9.4(,)]TJ T* [(USA\))-303.3(before)-299.9(being)-301.6(subjected)-306.3(to)-302(MS)-300(identi)]TJ /F12 1 Tf 16.8646 0 TD ()Tj /F11 1 Tf 0.5311 0 TD (cation.)Tj /F1 1 Tf 11.9999 0 0 11.9999 45.3543 388.4597 Tm [(LC-MS/MS)-305.3(Analysis)]TJ /F11 1 Tf 9.5 0 0 9.5 45.3543 376.9511 Tm [(Proteomic)-254.2(analyses)-259.9(were)-257.5(performed)-254.9(using)-262(an)-251.3(EASY-nLC)]TJ /F19 1 Tf 9 0 0 9 259.7102 379.9558 Tm ()Tj /F11 1 Tf 9.5 0 0 9.5 270.9354 376.9511 Tm (1200)Tj -23.7453 -1.2114 TD [(UHPLC)-300.8(system)-303.6(\(ThermoFisher)-302.7(Scienti)]TJ /F12 1 Tf 15.5636 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(c,)-302.9(Germany\))-301.3(coupled)-307.3(to)]TJ -16.0948 -1.2055 TD [(an)-233.4(Or)16.9(bi)20(tr)15.5(ap)-230.6(Q)-239.7(E)0(x)15.5(a)0(c)17.5(t)0(i)13(v)0(e)-230(H)0(F)14.9(-)0(X)-230.3(m)0(a)19.7(s)0(s)-232.8(s)0(p)12.7(e)0(c)20.4(t)0(r)15.5(o)0(m)17(e)0(t)13.8(e)0(r)-228.5(\()0(T)14.1(h)0(e)16(r)0(m)12.3(o)0(F)17.4(i)0(s)15.3(h)11.8(er)16.1(\))]TJ 0 -1.2114 TD [(op)17.4(er)16.1(at)16.9(in)13.3(g)-293.9(i)0(n)-285.1(t)0(h)15.4(e)-294.1(da)14.1(ta)16.9(-d)14.7(ep)17(en)14.1(de)17(nt)-290.9(ac)17.5(qu)15.5(is)15.3(it)18.9(io)14(n)-294.5(\()0(D)15.7(D)0(A)13.6(\))-295.5(m)12.3(o)0(d)11.5(e)-294.1(wh)20.4(ic)13.6(h)]TJ T* [(wa)15.9(s)-292.4(c)0(a)17.5(r)0(r)11.9(i)0(e)19.6(d)-291.6(ou)11.5(t)-288.8(a)0(s)-285.1(p)0(r)18.7(e)0(v)14.7(i)0(o)14(u)0(s)18.7(l)0(y)-288.6(d)12.7(esc)20.4(ri)15.3(be)14.9(d)-291.6(\()]TJ 0 0 0 0.7 k 16.1306 0 TD [(Pa)14.3(ng)-284.1(et)-284.6(al)18.6(.,)-286.1(20)15.1(2)13.5(0)]TJ 0 0 0 1 k 6.5405 0 TD (\).)Tj /F1 1 Tf 11.9999 0 0 11.9999 45.3543 308.4661 Tm [(Data)-301.6(Processing)]TJ /F11 1 Tf 9.5 0 0 9.5 45.3543 296.9574 Tm [(T)-6.6(h)0(e)-521(r)0(e)-7.7(s)0(u)-11.2(l)-6.6(ti)-10.9(ng)-522.8(MS)-520.8(ra)-10.6(w)-516.5(d)0(a)-9.8(t)0(a)-520.2(w)0(e)-11(r)0(e)-521(p)0(r)-11.2(o)-7.2(ce)-9.4(ss)-12(ed)-520.1(us)-11.2(in)-10.6(g)-514.7(P)0(r)-10.9(o)-7.3(te)-10.1(om)-6.9(e)]TJ 0 -1.2114 TD 0.0287 Tc [(Discover)4.8(er2)6.4(.)-3.9(2)-530.7(s)-1.2(oftware)-534(f)3.4(or)-532.3(database)-534(retrieval)-532.9(a)0.2(nd)-531.4(protein)]TJ T* 0 Tc [(qua)16.9(nt)13.5(i)]TJ /F12 1 Tf 2.5363 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ca)11.6(ti)13(on)14.6(.)-301.2(T)0(a)12.6(n)0(d)10.7(e)0(m)-299.7(m)0(a)13.7(s)0(s)-292.5(spe)16.9(ctr)13.8(a)-297(wer)12.8(e)-300.1(c)0(o)14.9(mpa)14.5(red)-287.4(ag)11.8(ain)14.6(st)-294.8(th)15.4(e)]TJ -3.0614 -1.2055 TD [(Uni)20.2(pro)17.4(t_)]TJ /F15 1 Tf 3.6701 0 TD [(Vi)11(br)13.5(io)-300.4(al)11.9(gi)11.7(no)14.3(lyt)17.2(ic)12.4(us)]TJ /F11 1 Tf 7.925 0 TD [(pr)12.7(ot)14.3(ein)-292.8(dat)17.7(ab)12(ase)-292.8(\(4,)13.6(33)15.1(8)-302.8(seq)12.9(ue)11(nc)14.1(es\))19(.)]TJ -11.5952 -1.2114 TD [(Tr)11.2(yp)11.2(sin)-344.8(was)-342.1(spe)11(ci)]TJ /F12 1 Tf 7.2149 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ed)-353(as)-350.8(a)-356.7(c)0(l)15.5(e)0(a)11.6(v)0(a)11.8(g)0(e)-355.3(e)0(n)14.1(zym)15.1(e)-353.8(a)0(l)12.7(low)18.6(in)13.3(g)-359.6(u)0(p)-350.5(t)0(o)-349.8(t)0(w)12.2(o)]TJ -7.74 -1.2114 TD [(mis)15.7(si)15.3(ng)-194.5(cl)15.5(ea)11.6(va)11.8(ges)14.6(.)-199.7(T)0(h)17.1(e)-198.7(pr)12.7(ecu)15.2(rs)11.9(or)-192.3(an)11.2(d)-196.1(fra)17.8(gme)15(nt)-189.4(io)14(n)-199(mas)13.7(s)-197(t)0(o)14.3(ler)15.5(an)11.2(ce)]TJ T* [(we)12.8(re)-294.2(se)16.1(t)-300.7(t)0(o)-290.1(1)0(0)-289.2(p)0(p)13.6(m)-298(an)11.2(d)-297.6(0.0)18.3(2)-296.8(Da.)-286.9(Ca)12.8(rba)17.9(mid)16.5(ome)21.2(thy)13.9(la)12.7(ti)13(on)-289.8(on)-289.8(Cys)]TJ T* [(was)-216.8(sp)12.7(ec)14.4(i)]TJ /F12 1 Tf 3.6403 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ed)-221.7(as)-219.5(a)]TJ /F12 1 Tf 2.8406 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(xe)12.7(d)-231.9(m)0(o)17(d)0(i)]TJ /F12 1 Tf 3.688 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ca)11.6(ti)13(on)-224.1(an)11.2(d)-226(s)0(u)12.7(c)0(c)14.4(iny)11.8(la)12.7(ti)13(on)-218.2(on)-218.2(pro)17.4(tei)17.2(n)]TJ -11.7443 -1.2114 TD 0.0157 Tc [(N)-3.2(-)5.7(t)-4.6(e)2(rminals)-521.4(w)0.5(ere)-523.1(s)-2.2(peci)]TJ /F12 1 Tf 10.0555 0 TD 0 Tc ()Tj /F11 1 Tf 0.5431 0 TD 0.0163 Tc [(ed)-520(as)-520.8(variable)-522.5(modi)]TJ /F12 1 Tf 8.7725 0 TD 0 Tc ()Tj /F11 1 Tf 0.549 0 TD 0.0145 Tc [(cat)-5.8(i)0(on)-5.5(s.)-525.4(False)]TJ -19.92 -1.2114 TD 0 Tc [(dis)16.1(co)14.9(ver)14.6(y)-443.1(r)0(a)13.3(t)0(e)-439.7(\(FD)16.5(R\))-439.4(thr)15.4(esh)16(ol)16(ds)-440.8(for)-432.4(pe)11(pti)13.8(de)-436.6(an)11.2(d)-446.8(p)0(r)12.7(o)0(t)14.3(ein)-436(we)12.9(re)]TJ 0 -1.2055 TD [(sp)12.7(eci)]TJ /F12 1 Tf 1.9455 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ed)-370.9(at)-365(0.0)18.3(5.)-371.2(Mi)17.1(nim)13.7(um)-368.8(pe)11(pt)16.4(ide)-361.6(len)13.5(gt)14(h)-370.1(was)-366(se)16.1(t)-378.3(a)0(t)-365(7)0(.)-365.2(Lys)16.2(in)13.3(e)]TJ -2.4706 -1.2114 TD [(su)12.7(cci)17.8(nyl)13.7(at)16.9(ion)-310.3(si)15.3(tes)-308.5(we)12.8(re)-312.1(ide)14.4(nt)13.5(i)]TJ /F12 1 Tf 11.9532 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(ed)-311.2(wi)12(th)-312.8(a)-314.9(loc)20.2(ali)16(za)11.6(ti)13(on)-313.7(pr)12.7(oba)16.7(bi)14(lit)18.3(y)]TJ -12.4784 -1.2114 TD [(set)-320.4(as)-320.9(>0)11.5(.75)18.3(.)-331(T)0(h)11.1(e)-330(mas)13.7(s)-328.2(s)0(p)12.7(ect)18(rom)17(et)13.8(ry)-329.8(pr)12.7(ot)14.3(eom)15.3(ic)13.6(s)-328.2(dat)17.7(a)-332.8(h)0(a)13.2(v)0(e)-325.5(bee)19.1(n)]TJ T* 0.0458 Tc [(dep)4.8(o)-3.2(s)4(i)1.4(ted)-550.2(t)1.7(o)-546.3(t)1.7(he)-546.7(Pr)4(oteom)4.4(e)-3.7(X)4.5(c)2.3(ha)5.4(nge)-546.7(C)3.6(ons)4(o)2.7(rtiu)4.8(m)-550.6(\()0.9(http)4.8(://)]TJ T* 0 Tc [(prot)-8.8(e)-7.7(ome)-8.6(centr)-13.9(al.)-8.1(prot)-8.8(eo)-9(mexch)-12.6(ang)-8.2(e.or)-11.8(g\))]TJ /F15 1 Tf 16.3633 0 TD [(vi)-9.1(a)]TJ /F11 1 Tf 1.7187 0 TD [(the)-511.5(iP)-7.5(ro)-7.3(X)-512.7(par)-9.8(tner)]TJ -18.082 -1.2114 TD [(rep)16.9(osi)20(tor)14.2(y)-293.9(w)0(i)12(t)0(h)-283(the)-284.7(da)14.1(tas)16.9(et)-290.5(ide)20.4(nti)]TJ /F12 1 Tf 13.1647 0 TD ()Tj /F11 1 Tf 0.5252 0 TD [(er)-288.2(PXD)14.4(02)15.1(315)16.7(3.)]TJ /F1 1 Tf 11.9999 0 0 11.9999 45.3543 102.274 Tm (Co-Immunoprecipitation)Tj 0 -1.0441 TD [(and)-300.9(Western)-305.4(Blotting)]TJ /F11 1 Tf 9.5 0 0 9.5 45.3543 78.2929 Tm 0.0881 Tc (Speci)Tj /F12 1 Tf 2.5184 0 TD 0 Tc ()Tj /F11 1 Tf 0.6206 0 TD 0.088 Tc [(c)-588.1(p)-0.7(olyclonal)-587(a)-0.2(ntibodies)-586.4(t)2.1(o)-587.6(S)1(odB)-582.2(a)-0.2(nd)-585.5(PEPCK)]TJ -3.139 -1.2114 TD 0 Tc [(\(Phosphoenolpyruvate)-419.8(carboxykinase,)-416(one)-404.9(of)-414.5(the)-410(key)-409.4(enzymes)]TJ 27.4512 67.7568 TD [(in)-511.9(gl)-8.1(uco)-14.1(ne)-9.8(og)-8.8(ene)-11.5(si)-8.6(s)-507.3(p)0(a)-9.8(t)0(h)-8.4(w)0(a)-7.9(y)0(\))-511.9(w)0(e)-11(r)0(e)-509(u)0(s)-11.2(e)0(d)-514.1(t)0(o)-510.9(p)0(r)-11.2(e)0(c)-9.4(i)0(p)-7.7(ita)-9.5(te)-511.3(t)-8.3(arg)-12.1(et)]TJ 0 -1.2114 TD 0.0215 Tc [(pr)3.6(oteins)3.6(.)]TJ /F15 1 Tf 4.1953 0 TD 0.0206 Tc [(V.)-520.2(alginolyt)-5.6(i)4.2(cus)]TJ /F11 1 Tf 7.1433 0 TD [(strain)-524.6(cell)-523.2(l)2(ysates)-522.6(\(500)-521(g\))-519.7(w)-0.7(e)-5.1(r)2.6(e)]TJ -11.3385 -1.2114 TD 0 Tc [(interacted)-448.1(with)-444(SodB)-447.4(and)-441.5(PEPCK)-447.3(antibody)-444.5(at)-448.6(4C)-441.7(overnight.)]TJ T* [(Protein)-450.6(A/G)-449.3(beads)-454.4(washed)-450.6(three)-447.6(times)-453.9(with)-450(PBS)-445.9(buf)57.8(fer)-450.8(were)]TJ T* [(added)-499.2(to)-499(the)-499.5(lysates)-494.3(at)-502.3(4C)-495.4(for)-498(1)]TJ /F17 1 Tf 14.382 0 TD ()Tj /F11 1 Tf 0.5132 0 TD 0.4937 Tc [(3h)-7.7(\()]TJ 0 0 0 0.7 k 2.3453 0 TD 0 Tc [(Cheng)-501.2(et)-499.4(al.,)-500.2(2019)]TJ 0 0 0 1 k 7.8594 0 TD (\).)Tj -25.1 -1.2114 TD [(The)-414.3(beads)-412.7(were)-418.6(pelleted)-413(at)-418.7(4C,)-414.6(followed)-415.3(by)]TJ /F12 1 Tf 18.8638 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(ve)-415(washes)-415.6(with)]TJ -19.3949 -1.2055 TD [(PBS)-296.7(buf)63.8(fer.)-298.4(Then)-297(50)]TJ /F14 1 Tf 8.3965 0 TD (m)Tj /F11 1 Tf 0.5729 0 TD [(l)-299(o)0(f)-295.1(loading)-298.3(sample)-298.2(buf)63.8(fer)-295.6(\(250)-296.7(mM)-296.2(Tric-)]TJ -8.9694 -1.2114 TD [(HCl)-285.4(pH)-283.5(=)-287.3(6.8,)-282.9(10%)-285.2(SDS,)-283.3(0.5%)-288(bromophenol)-289(blue,)-286.1(50%)-279.2(glycerol,)]TJ T* [(and)-513.1(5%)]TJ /F14 1 Tf 3.7477 0 TD (b)Tj /F11 1 Tf 0.549 0 TD [(-me)-11.4(rcap)-11.5(toe)-11.3(tha)-7.1(n)-8(ol\))-512.3(was)-509.2(ad)-9.8(ded)-513.3(to)-510.9(th)-8.4(e)-503(pel)-13.5(let)-10.7(,)-504.1(b)0(o)-8.5(iled)]TJ -4.2967 -1.2114 TD 0.0204 Tc [(for)-522.7(5)-521.1(min,)-519.5(and)-521.8(s)-3.5(ubse)-5.2(quen)-5.5(tly)-524.2(a)3.9(nalyzed)-521.8(b)-4.8(y)-518.2(SDS-PAGE)-521.6(and)]TJ T* 0 Tc [(Western)-305.1(blotting.)]TJ 1.2651 -1.2114 TD [(For)-370.5(Western)-382.7(blotting,)-374.9(proteins)-379.3(were)-376.8(run)-371.3(on)-379.3(12%)-374.7(1-DE)-370.5(gels)]TJ -1.2651 -1.2055 TD [(and)-369.9(transferred)-371.3(to)-367.7(a)-368.6(polyvinylidene)]TJ /F12 1 Tf 14.8117 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(uoride)-374(\(PVDF,)-368.4(Millipore,)]TJ -15.3428 -1.2114 TD [(Billerica,)-210.2(MA,)-209.2(USA\))-213.8(membrane.)-210.4(The)-211.4(membranes)-207.6(were)-209.7(blocked)-215.1(in)]TJ T* [(Tris)-212.2(buf)63.8(fered)-213(saline)-214.6(\(TBS,)-208.8(500)-210.1(mM)-206.7(Tris-HCl;)-218(2.8)-208.5(M)-207.1(NaCl;)-212.4(60)-211.7(mM)]TJ T* [(KCl;)-412.1(pH7.4\))-417.6(containing)-410.7(0.05%)-417.7(\(v/v\))-409.4(Tween)-415.3(20)-414.6(with)-414.2(5%)-406.1(\(w/v\))]TJ T* [(skimmed)-307.4(milk)-301.6(and)-304.2(incubated)-307.2(for)-301.1(1)-302.8(h)-298.5(at)-305.3(room)-300.6(temperature.)-307.8(The)]TJ T* [(primary)-502.9(antibodies)-501.1(used)-501.4(in)-500(the)-499.5(western)-500.6(blot)-494.9(were)-502.1(anti-SodB)]TJ 0 -1.2055 TD [(\(1:4,000\),)-352.9(anti-PEPCK)-355.5(\(1:4,000\),)-352.9(and)-352(anti-succinyllysine)-356(mouse)]TJ 0 -1.2114 TD [(mAb)-328.4(\(PTM)-329.2(Biolabs)-328.6(Inc.,)-332.9(Hangzhou,)-333.5(China\))-329.2(\(1:5m000)-325.8(in)-332.9(TBST)]TJ T* 0.0245 Tc [(w)3.3(i)4(th)-518.7(5%)-520.5(sk)6.2(imm)7(e)-1.1(d)-517.7(m)1(il)6(k\))-521.7(an)4.5(d)-523.7(i)4(nc)4.9(ubat)4.2(e)4.9(d)-523.7(o)5.3(v)-0.9(e)4.9(r)0.6(ni)4(gh)6.5(t)-520.9(a)2(t)-520.9(4)2.2()5.4(C)0.2(.)]TJ T* 0 Tc [(Horseradish)-305.9(peroxidase)-306.2(\(HRP\))-308.1(conjugated)-300.8(goat)-302.2(anti-mouse)-309.9(IgG)]TJ T* [(\(H+L\))-333.4(\(Beyotime)-338(Biotechnology,)-333.1(Shanghai,)-338.2(China\))-329.2(was)-336.2(used)-334.3(as)]TJ T* [(the)-314.5(secondary)-316.5(antibody)-319.2(at)-317.3(a)-314.9(1:10,000)-312.4(dilution)-320.2(in)-315(TBST)-316.9(with)-312.7(3%)]TJ T* [(skimmed)-337.3(milk.)-340.2(Finally,)-336.3(the)-338.4(membrane)-338.9(was)-336.2(visualized)-342(using)-339.6(the)]TJ 0 -1.2055 TD [(ECL)-268.1(system)-267.8(\(Bio-Rad,)-270.8(Hercules,)-268.4(CA,)-265.1(USA\),)-270.3(and)-268.4(recorded)-267.5(by)-271.3(the)]TJ 0 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[(accounts)-490.4(for)-492.1(24,)-489(33,)-494.9(and)-489.2(34%)-488.1(of)-492.1(the)-487.6(total)-488.7(succinyl)-492.9(proteins,)]TJ T* [(respectively)-498.2(\()]TJ /F20 1 Tf 0 0 0 rg 5.4843 0 TD [(Figure)-492.7(2)]TJ /F11 1 Tf 0 0 0 1 k 3.6999 0 TD [(\).)-489.3(Moreover,)-498(other)-494.9(molecular)-492.9(functions)]TJ -9.1842 -1.2055 TD [(in)-10.6(c)-7.7(l)0(u)-11.8(d)0(e)-514.1(s)0(m)-11.6(a)0(l)-11.2(l)-507.9(mo)-12.8(le)-8.3(c)-7.7(ule)-520.8(bi)-9.8(ndi)-15.8(ng)-9.5(,)-510.1(i)-8.5(on)-510.6(b)-7.2(i)0(n)-10.6(d)0(i)-13.7(n)0(g)-9.5(,)-510.1(an)-12.6(d)-506.4(s)0(t)-14.3(r)0(u)-11.2(c)0(t)-10.1(u)0(r)-11.2(a)0(l)]TJ 0 -1.2114 TD [(constituents)-447.4(of)-438.3(ribosomes,)-440.3(representing)-444.7(22,)-441.2(12,)-441.2(and)-441.5(8%)-441.9(of)-438.3(all)]TJ 27.4512 46.0524 TD (identi)Tj /F12 1 Tf 2.3035 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(ed)-478.3(proteins,)-477.6(respectively)-486.2(\()]TJ /F20 1 Tf 0 0 0 rg 10.8432 0 TD [(Figure)-480.7(2A)]TJ /F11 1 Tf 0 0 0 1 k 4.3862 0 TD [(\).)-477.4(The)-479.9(other)-477(large)]TJ -18.0641 -1.2114 TD [(groups)-377.2(in)-380.6(terms)-373.7(of)-378.7(biological)-376.2(processes)-381.8(are)-376.4(proteins)-379.3(associated)]TJ T* 0.0123 Tc [(with)-507(organo)5(nitrogen)-508.9(compound)-512(metabolic)-514.6(proce)4.6(sses)-512.9(\()-2.8(14%\),)]TJ T* 0 Tc [(or)-7.3(gan)-8.2(i)-8.5(c)-503(su)-11.2(bs)-7.3(ta)-7(nce)-512.8(me)-7.3(ta)-7(bo)-8.5(lic)-512.2(pro)-12.4(ces)-9.5(se)-7.7(s)-507.3(\()0(1)-7.5(3)0(%)-6.3(\))0(,)-513.2(and)-513.1(me)-7.3(ta)-7(bo)-8.5(lic)]TJ T* [(processes)-346(\(8%\))-340.8(\()]TJ /F20 1 Tf 0 0 0 rg 6.6659 0 TD [(Figure)-343.5(2B)]TJ /F11 1 Tf 0 0 0 1 k 4.1833 0 TD [(\).)-340.1(Cell)-343.6(\(11%\),)-342(other)-339.7(cell)-338.9(components)]TJ -10.8492 -1.2114 TD [(\(7%\),)-415.2(intracellular)-426.7(\(3%\),)-415.2(and)-417.6(macromolecular)-422.6(complexes)-421.2(\(1%\))]TJ 0 -1.2055 TD [(a)-10.6(r)0(e)-526.9(c)-7.7(l)-6.6(a)-10.6(ss)-18(i)]TJ /F12 1 Tf 3.8969 0 TD ()Tj /F11 1 Tf 0.5371 0 TD 0.0081 Tc [(e)-5.6(d)-504.3(in)-513.1(cel)-4.5(lular)-505.1(c)-5.6(om)8.1(p)-8.6(o)0.9(ne)-5.6(nts)-505.1(\()]TJ /F20 1 Tf 0 0 0 rg 12.1322 0 TD 0.0097 Tc [(Fig)9.7(u)-5.6(re)-506.2(2C)]TJ /F11 1 Tf 0 0 0 1 k 4.4519 0 TD 0.0079 Tc [(\).)-514.1(Th)7.9(e)-519.1(G)-0.2(O)]TJ -21.0181 -1.2114 TD 0.0187 Tc [(analysis)-518.4(of)-519.8(th)-5.3(e)-514.2(s)-5.2(uccinylome)-520.1(suggests)-518.4(t)-1.6(hat)-514.8(t)-1.6(he)-520.1(su)-4.4(ccinylated)]TJ T* 0 Tc [(proteins)-367.3(are)-370.4(related)-368.4(to)-361.7(dif)56.5(ferent)-367.4(molecular)-367.5(functions,)-373.6(biological)]TJ T* 0.0178 Tc [(proces)-6.1(ses,)-522.1(and)-518.5(c)4.1(ellular)-519.3(c)-1.8(omponents)-6.1(,)-516.1(and)-518.5(c)-1.8(l)5.2(o)-1.4(sely)-514.8(related)-518.5(t)3.5(o)]TJ T* 0 Tc [(bacterial)-300.1(life)-304.8(activities.)]TJ 1.2651 -1.2114 TD [(The)-283(KEGG)-286.1(analysis)-290.6(of)-283.2(the)-284.7(succinylated)-291.9(proteins)-283.8(showed)-286.2(that)]TJ -1.2651 -1.2055 TD [(most)-409.1(identi)]TJ /F12 1 Tf 4.6906 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(ed)-412.7(proteins)-415.1(were)-412.6(enriched)-409(in)-416.4(metabolic)-409.7(pathways)]TJ -5.2217 -1.2114 TD [(\(17%\),)-419.6(biosynthesis)-421.4(of)-414.5(antibiotics)-417.3(\(15%\),)-419.6(ribosomes)-419.6(\(8%\),)-421.2(and)]TJ T* [(29%)-440.4(succinylated)-441.1(protein)-444.9(were)-436.5(not)-441.3(enriched)-438.9(in)-440.3(the)-439.8(metabolic)]TJ T* [(pathway)-338(category)-337(\()]TJ /F20 1 Tf 0 0 0 rg 7.7341 0 TD [(Figure)-337.5(2D)]TJ /F11 1 Tf 0 0 0 1 k 4.3027 0 TD [(\).)-334.2(In)-337.4(this)-333.3(study,)-339.3(we)-339.2(found)-337.3(that)-331.7(54)]TJ -12.0368 -1.2114 TD 0.0431 Tc [(ribosomal)-542.4(proteins)-541.8(were)-537.5(succin)5.2(y)-0.2(lated,)-538.6(including)-543.2(2)2.9(1)-546.1(30S)]TJ T* 0 Tc [(ribosomal)-333.6(proteins)-337.5(and)-340(33)-331(50S)-334.5(ribosomal)-339.6(proteins)-337.5(were)-335(related)]TJ 0 -1.2055 TD [(to)-218.5(translation)-225.5(processes.)-229.4(Interestingly,)-225.5(succinylation)-231.2(of)-217.5(ribosomal)]TJ 0 -1.2114 TD [(proteins)-379.3(was)-372(also)-376.6(found)-373.1(in)]TJ /F15 1 Tf 11.3445 0 TD [(M.)-376(tuberculosis)]TJ /F11 1 Tf 6.1049 0 TD (,)Tj /F15 1 Tf 0.5908 0 TD [(A.)-374.2(hydrophila)]TJ /F11 1 Tf 5.526 0 TD [(,)-378.8(and)]TJ /F15 1 Tf -23.5663 -1.2114 TD [(E.)-299.1(coli)]TJ /F11 1 Tf 2.7332 0 TD (\()Tj 0 0 0 0.7 k 0.3461 0 TD [(Colak)-299.8(et)-302.5(al.,)-303.3(2013)]TJ 0 0 0 1 k 6.9941 0 TD (;)Tj 0 0 0 0.7 k 0.5192 0 TD [(Xie)-302.2(et)-296.5(al.,)-303.3(2014)]TJ 0 0 0 1 k 5.9796 0 TD (;)Tj 0 0 0 0.7 k 0.5192 0 TD [(Yao)-299.9(et)-302.5(al.,)-303.3(2019)]TJ 0 0 0 1 k 6.2243 0 TD (\).)Tj -22.0505 -1.2114 TD [(The)-521.7(C)-6.4(O)0(G)-522.3(i)0(s)-521.8(a)-517.8(too)-10.9(l)-513.9(f)-7.4(or)-520.5(ge)-9.2(nom)-8.9(e-sca)-12.2(le)-521.6(ana)-11.3(ly)-8.1(sis)-521.8(of)-521.9(pr)-11.2(otei)-8(n)]TJ -1.2651 -1.2114 TD [(fu)29.2(nc)26(ti)24.9(on)26.5(s)-483.4(a)13.3(n)15.9(d)-482.6(e)16.2(v)0(o)27(l)0(u)24(t)15.5(i)15.3(o)0(n)26.5(.)-480.2(In)-468.7(t)15.5(h)11.8(is)-468.1(s)11.9(t)15.6(u)12.7(d)12.7(y)0(,)-469.8(C)17.5(OG)-468.6(a)13.3(n)15.9(a)13.3(l)0(y)21.7(s)17.9(is)-468.1(r)11.9(e)0(v)26.6(e)0(a)23.5(l)17.3(ed)]TJ T* 0.0277 Tc [(that)-541.6(translation,)-542(ribosomal)-539.9(s)-2.2(truc)-3.9(ture)-546.9(and)-544.4(b)2.5(ioge)-3.9(ne)-3.9(sis)-539.3(\()-5.3(134)]TJ T* 0 Tc [(suc)11(ciny)10(late)14.5(d)-512.4(pro)11.4(tei)11.2(ns\),)-503.3(ami)11.1(no)-504.6(acid)-497.5(tra)10.9(nspor)15.3(t)-509.6(and)-501.2(meta)15.6(boli)12.1(sm)]TJ 0 -1.2055 TD -0.0143 Tc [(\(97\))-14.3(,)-294.7(p)-1.5(ostt)-14.3(r)7.2(a)-1(nsla)-14.3(t)8.6(i)1(o)-14.3(n)12.2(al)-307.4(modi)]TJ /F12 1 Tf 10.885 0 TD 0 Tc ()Tj /F11 1 Tf 0.5192 0 TD [(ca)23.5(t)15.6(i)15.3(on)26.5(,)-295.2(p)12.7(r)17.9(o)0(t)26.2(e)0(i)25.5(n)-294.5(t)15.6(u)12.7(r)11.9(n)15.9(ov)27(er)28.1(,)-295.2(c)16.2(h)11.8(a)13.3(p)12.7(e)0(r)28.1(o)0(n)26.5(e)0(s)]TJ -11.4042 -1.2114 TD [(\(62)18(\),)-495.3(an)11.2(d)-500.5(gen)12.6(era)17.5(l)-501.9(f)0(u)11.3(n)0(c)14.1(t)0(i)13(o)0(n)-498.7(p)0(r)12.7(e)0(d)11(ict)17.2(io)14(n)-503.3(mec)14.8(ha)13.2(ni)13.3(sms)-495(\(5)16.4(1\),)-493.7(we)12.8(re)]TJ T* 0.0239 Tc (signi)Tj /F12 1 Tf 1.9992 0 TD 0 Tc ()Tj /F11 1 Tf 0.555 0 TD 0.025 Tc [(cant)-532.3(\()]TJ /F20 1 Tf 0 0 0 rg 2.6974 0 TD 0.0219 Tc [(Figure)-541.7(3A)]TJ /F11 1 Tf 0 0 0 1 k 4.6249 0 TD 0.0247 Tc [(\).)-533.1(Our)-536.3(r)0.8(esult)4.4(s)-536.3(were)-538(c)5.1(o)-0.5(nsi)4.2(s)0.8(te)5.1(nt)-538.6(wi)4.2(th)]TJ -9.8765 -1.2114 TD 0.0321 Tc [(previ)5.6(o)1(us)-540.8(succinyl-proteo)6.9(me)-542.5(studies)-540.8(c)0.5(onducted)-540(in)]TJ /F15 1 Tf 22.5756 0 TD 0.033 Tc [(E.)-543.6(co)5.7(li)]TJ /F11 1 Tf 2.8645 0 TD 0 Tc (,)Tj /F15 1 Tf -25.4401 -1.2114 TD [(M)15.7(.)-302.1(tu)21.4(b)13.5(e)13.1(r)17.9(c)0(u)22.8(l)18.2(os)25.2(i)13.4(s)]TJ /F11 1 Tf 5.8543 0 TD (,)Tj /F15 1 Tf 0.5192 0 TD -0.0129 Tc [(B.)-315(s)-12.9(u)9.7(btilis)]TJ /F11 1 Tf 3.7417 0 TD 0.3072 Tc [(,a)320.5(n)323.1(d)]TJ /F15 1 Tf 2.2975 0 TD 0 Tc [(V.)-292.6(p)13.5(a)11.6(r)11.9(a)17.6(ha)26.3(e)13.1(m)0(o)25.6(l)12.2(y)13.3(t)15.6(ic)24.4(u)11.8(s)]TJ /F11 1 Tf 8.2413 0 TD (\()Tj 0 0 0 0.7 k 0.3342 0 TD [(C)17.5(o)0(l)21.9(a)13.3(k)-298.7(e)0(t)-290.5(a)13.3(l.)26.4(,)]TJ -20.9882 -1.2114 TD -0.0135 Tc (2013)Tj 0 0 0 1 k 1.8619 0 TD 0 Tc (;)Tj 0 0 0 0.7 k 0.4297 0 TD -0.0144 Tc [(Ko)-14.4(s)8.2(o)2.2(n)-14.4(o)-214.7(et)-225.6(al)-14.4(.)12(,)-226.1(2015)]TJ 0 0 0 1 k 7.2865 0 TD 0 Tc (;)Tj 0 0 0 0.7 k 0.4297 0 TD -0.0144 Tc [(Pan)-225.3(e)-14.4(t)-215.4(a)-1.1(l.)-14.4(,)-216.9(2015)]TJ 0 0 0 1 k 5.8006 0 TD 0 Tc (;)Tj 0 0 0 0.7 k 0.4297 0 TD -0.0145 Tc [(Yang)-230.8(et)-225.7(al)-14.5(.)11.9(,)-226.2(2015)]TJ 0 0 0 1 k 6.3018 0 TD [(\),)-226.1(whic)-14.4(h)]TJ -22.5398 -1.2055 TD 0 Tc [(re)-13.7(v)-7.5(e)-13.7(al)-11.2(e)-7.7(d)-530.3(t)-8.3(h)-12.1(at)-532.1(t)-8.3(h)-12.1(e)-526.9(m)0(a)-16.1(j)-6.6(o)-7.3(r)-12(i)-8.5(t)-8.3(y)-526.7(o)-7.3(f)-532.5(s)0(u)-11.2(c)-13.7(c)-7.7(i)-8.5(n)-8(y)-7.5(l)-6.6(-)-10(pr)-11.2(o)-13.2(t)0(e)-16(i)0(n)-10.6(s)-531.1(c)-7.7(o)-7.3(n)-8(s)-12(i)-8.5(s)0(t)-14.3(e)-7.7(d)-530.3(o)-7.3(f)]TJ 0 -1.2114 TD [(t)15.5(r)11.9(a)13.3(n)0(s)27.7(l)0(a)24.6(t)15.5(io)26(n)-288.5(a)13.3(n)15.8(d)-291.6(m)18.3(e)0(t)25.7(a)13.3(bo)27.3(li)26.6(c)-288.2(p)12.7(r)11.9(o)16.6(te)25.8(i)15.3(n)0(s)21.8(.)]TJ 1.2651 -1.2114 TD [(The)-402.4(domain)-403.2(is)-402.4(the)-404(structural)-404(basis)-402.4(of)-402.5(protein)-403.1(physiological)]TJ -1.2651 -1.2114 TD [(function,)-236.3(thus)-234.4(in)-231.4(order)-235(to)-230.4(further)-237.6(identify)-234.2(the)-237(function)-233.5(associated)]TJ T* [(with)-479.8(succinylation,)-484.7(the)-481.6(domain)-486.7(of)-480.1(the)-481.6(identi)]TJ /F12 1 Tf 18.8041 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(ed)-484.3(succinylated)]TJ -19.3352 -1.2114 TD 0.0118 Tc [(proteins)-513.4(were)-509.1(a)-4.7(n)-2.2(notated.)-516.2(The)-509.1(r)-6.1(es)-6.1(ults)-513.4(s)-6.1(h)-0.3(own)-515.4(i)3.3(n)]TJ /F20 1 Tf 0 0 0 rg 20.9882 0 TD [(Figu)-3(re)-515.9(3B)]TJ /F11 1 Tf 0 0 0 1 k 4.4519 0 TD 0 Tc (,)Tj -25.4401 -1.2114 TD 0.0363 Tc [(i)3.9(n)4.4(di)3.9(cat)4.1(e)-532.4(e)4.7(n)4.4(ri)3.9(ch)6.3(ed)-529.8(s)6.4(u)1.3(cc)4.7(i)3.9(n)4.4(yl)5.8(at)4.1(ed)-529.8(su)7.3(bs)6.4(tr)6.4(at)4.1(es)-530.7(wi)3.9(t)4.1(h)-530.8(f)5(u)1.3(nc)4.7(t)4.1(i)3.9(on)4.4(al)]TJ 0 -1.2055 TD 0.0322 Tc [(domains)-528.8(i)-0.2(ncluding)-530.3(NAD\(P\)-binding)-536.2(domain)-530.8(superfamily,)]TJ ET 0 0 0 0.7 k 45.354 246.161 m 549.921 246.161 l 549.694 245.934 l 45.581 245.934 l f* 549.921 246.161 m 549.921 66.557 l 549.694 66.784 l 549.694 245.934 l f* 549.921 66.557 m 45.354 66.557 l 45.581 66.784 l 549.694 66.784 l f* 45.354 66.557 m 45.354 246.161 l 45.581 245.934 l 45.581 66.784 l f* /EmbeddedDocument /MC7 BDC /Document /MC8 BDC q 133.168 222.606 133.056 -119.765 re W n q 133.482056 0 0 120.42601 132.852005 102.492035 cm /Im1 Do Q Q q 265.935 222.606 133.199 -119.765 re W n q 133.205994 0 0 120.42601 265.932007 102.492035 cm /Im2 Do Q Q q 398.847 222.606 66.489 -119.765 re W n q 66.990059 0 0 120.425995 398.832031 102.492035 cm /Im3 Do Q Q q 129.94 228.416 335.395 -125.575 re W n BT /F21 1 Tf 7 0 0 7 129.989 221.2847 Tm 0 0.003922 0 rg 0 Tc (A)Tj ET Q BT /F21 1 Tf 7 0 0 7 260.6815 221.2847 Tm 0 0.003922 0 rg 0 Tc (B)Tj 16.8571 0.1887 TD (C)Tj ET EMC EMC BT /F1 1 Tf 6.9999 0 0 6.9999 50.6268 81.8078 Tm 0 0 0 1 k [(FIGURE)-299.4(1)]TJ /F7 1 Tf 5.0053 0 TD [(|)-429.9(Pro)]TJ /F8 1 Tf 2.1382 0 TD ()Tj /F7 1 Tf 0.4374 0 TD [(le)-301.1(of)]TJ /F6 1 Tf 2.0896 0 TD [(V.)-305.2(alginolyticus)]TJ /F7 1 Tf 6.5765 0 TD [(lysine)-300.3(succinylation)-308.4(proteome.)]TJ /F1 1 Tf 13.072 0 TD (\(A\))Tj /F7 1 Tf 1.555 0 TD [(Distributions)-310.1(of)-299.5(mass)-301.8(errors)-301.1(for)-295.3(lysine)-308.4(succinylated)-300.2(peptides.)]TJ /F1 1 Tf 25.8929 0 TD (\(B\))Tj /F7 1 Tf 1.5793 0 TD [(Distribution)-303.2(of)-299.5(lysine)]TJ -58.3462 -1.3607 TD [(succinylated)-300.1(peptides)-301.9(based)-305.1(on)-302(their)-301(length.)]TJ /F1 1 Tf 19.1625 0 TD (\(C\))Tj /F7 1 Tf 1.6198 0 TD [(The)-302.2(pie)-304.2(chart)-300.1(shows)-297.7(the)-304.1(distribution)-302.9(of)-299.5(succinylation)-308.4(sites)-299.4(in)-304.4(each)-295.9(protein.)]TJ ET 0 0 0 0.7 k 45.354 737.518 504.567 0.28345 re f BT 6.9999 0 0 6.9999 45.3543 744.2644 Tm [(Zeng)-305.3(et)-300.5(al.)]TJ 54.7663 0 TD [(Succinylation)-308.2(in)]TJ /F6 1 Tf 6.8924 0 TD [(V.)-305.2(alginolyticus)]TJ /F7 1 Tf 6.5765 0 TD (Virulence)Tj ET 45.354 42.803 504.567 0.22675 re f 45.354 42.803 504.567 0.22675 re f BT 6.9999 0 0 6.9999 45.3543 31.2945 Tm 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[(acet)13.4(yla)11.1(ted)-504.6(sit)12.9(es)-509(with)15.5(in)-505.9(1,1)12.3(78)-504.1(pro)11.4(teins)15.1(.)-510.1(I)0(n)-504.5(ord)11.4(er)-509(to)-504.9(de)11(termi)11.6(ne)]TJ 0 -1.2114 TD [(w)14.6(h)17.8(et)25.7(h)11.8(e)16.2(r)-453.6(s)11.9(u)12.7(c)16.2(c)16.2(i)15.3(ny)26.3(l)17.3(a)13.3(t)15.5(i)0(o)26(n)-455.6(a)13.3(n)15.8(d)-452.7(a)13.3(ce)26.4(t)15.5(y)16.4(la)24.6(t)15.6(i)15.3(o)16.6(n)]TJ /F17 1 Tf 15.7069 0 TD ()Tj /F11 1 Tf 0.3819 0 TD -0.0138 Tc [(crosstal)3.5(k)]TJ /F17 1 Tf 3.3598 0 TD 0 Tc ()Tj /F11 1 Tf 0.8474 0 TD [(o)16.6(c)0(c)26.4(u)12.7(r)11.9(s)-453.6(a)13.3(t)-449.9(th)27.4(e)]TJ -20.296 -1.2114 TD [(s)11.9(a)19.3(m)12.3(e)-234.5(l)17.3(y)0(s)28.3(i)15.3(ne)-224.6(s)17.9(i)15.3(te)25.7(,)-235.5(w)14.6(e)-228.5(c)0(o)26.8(m)12.3(p)12.8(a)19.3(r)11.9(e)16.2(d)-237.9(t)15.5(h)17.8(e)-234.5(ly)27.7(s)11.9(i)15.3(n)15.9(e)-234.5(s)11.9(u)18.7(cc)26.4(i)15.3(n)0(y)26.3(l)17.3(a)13.3(t)15.5(i)15.3(o)0(n)-224.1(d)18.7(a)13.3(t)15.6(a)-237.3(h)17.8(er)28.1(e)-234.5(t)15.5(o)]TJ 0 -1.2055 TD [(th)15.4(e)-503(p)0(r)18.7(evi)18(ou)11.5(s)-495.3(a)0(c)11.6(e)0(t)13.8(y)0(l)15.8(ati)20.3(on)-492.7(da)14.1(ta)-490.3(on)-492.7(po)11.5(st)15.5(-tr)17.4(an)17.2(sla)18.6(ti)18.9(on)14.6(al)12.7(ly)-491.5(mod)17.8(i)]TJ /F12 1 Tf 24.2048 0 TD ()Tj /F11 1 Tf 0.5252 0 TD (ed)Tj -24.73 -1.2114 TD [(p)12.7(r)17.9(ot)26.2(e)16.2(i)15.3(ns)-306.4(a)13.3(n)15.8(d)-321.4(p)12.7(e)16.2(p)12.7(t)15.6(i)15.3(d)12.8(e)16.2(s)-322.3(\()]TJ /F20 1 Tf 0 0 0 rg 9.1424 0 TD -0.0144 Tc [(Figures)-331.6(6)]TJ /F11 1 Tf 0 0 0 1 k 4.1296 0 TD -0.0146 Tc (and)Tj /F20 1 Tf 0 0 0 rg 1.7903 0 TD 0 Tc (7)Tj /F11 1 Tf 0 0 0 1 k 0.4834 0 TD -0.0141 Tc [(\).)-333.2(T)3.1(h)-2.3(e)-332.1(c)2.1(o)-14.1(m)14.8(parison)-332.4(r)-2.2(es)3.8(ul)-14.1(t)12.7(s)]TJ -15.5457 -1.2114 TD -0.0143 Tc [(sh)3.5(owe)-14.3(d)-438.9(that)-452.3(5)5.2(0)-0.8(2)-454.3(p)4.4(rot)-14.3(e)11.5(ins)-455.9(o)2.3(ve)-14.3(r)13.8(l)-14.3(a)16.3(pped)-455.1(\()]TJ /F20 1 Tf 0 0 0 rg 15.8262 0 TD -0.0147 Tc [(Figure)-453(6A)]TJ /F11 1 Tf 0 0 0 1 k 4.237 0 TD 0 Tc [(\),)-423.7(a)13.3(n)0(d)-424.9(f)16.5(u)12.8(r)11.9(t)15.5(h)11.8(e)16.2(r)]TJ -20.0633 -1.2114 TD [(e)16.2(n)0(r)27.8(i)15.3(ch)28(m)12.3(e)16.2(nt)-386.3(a)13.3(n)15.8(a)13.3(l)17.3(ys)28.3(is)-390.5(o)16.6(f)-401.2(K)15.5(E)13(G)15.8(G)-396(p)12.8(a)13.3(t)15.6(h)11.8(w)14.6(a)13.3(y)16.4(s)-399.9(w)14.6(a)13.3(s)-399.9(p)12.7(e)16.2(r)11.9(f)16.5(o)16.6(r)11.9(m)12.3(e)16.2(d)12.7(.)-396.7(O)0(f)-384.3(t)0(h)27.4(e)]TJ T* [(o)16.6(v)0(e)26.6(r)11.9(l)17.3(a)13.3(p)12.8(p)18.7(ed)-227.7(p)18.7(r)11.9(o)16.6(t)0(e)25.7(i)15.3(n)15.8(s)11.9(,)-235.5(a)-237.3(t)15.5(o)0(t)26.2(a)13.3(l)-233.4(o)16.6(f)-240.1(1)13.5(0)-231.2(K)0(E)22.5(G)15.8(G)-234.9(p)12.7(a)13.3(t)15.6(h)17.8(w)14.6(a)13.3(y)0(s)-222.3(a)13.3(r)11.9(e)-234.5(e)16.2(n)0(r)27.7(i)0(c)25.5(h)17.8(ed)22.9(,)-235.5(o)16.6(f)]TJ T* 0.0143 Tc [(which)-528.9(b)1.1(iosynthesis)-528.8(o)1.1(f)-530.2(a)-2.2(ntibiotics,)-531.6(r)2.3(ibosome)-5.3(,)-525.6(and)-527.9(m)-3.2(etabolic)]TJ 0 -1.2055 TD 0 Tc [(p)12.7(a)13.3(th)21.4(w)14.6(a)13.3(ys)-478.9(a)13.3(r)11.9(e)-491.1(d)0(o)23.4(m)12.3(in)19.2(a)13.3(n)15.8(t)-491.7(\()]TJ /F20 1 Tf 0 0 0 rg 10.4673 0 TD -0.014 Tc [(Fi)-14(g)8.1(u)0.9(re)-506(6)-14(B)]TJ /F11 1 Tf 0 0 0 1 k 4.243 0 TD 0 Tc [(\).)-477.4(Am)19(o)16.6(n)0(g)-481(t)15.6(h)11.8(e)-491.1(1)13.5(6)0(9)-480.2(s)11.9(p)12.7(e)0(c)20.4(i)]TJ /F12 1 Tf 10.0137 0 TD ()Tj /F11 1 Tf 0.5192 0 TD (c)Tj -25.2432 -1.2114 TD [(s)11.9(u)18.7(cc)26.4(i)15.3(n)0(y)26.3(l)17.3(a)13.3(t)15.5(ed)-353(m)12.3(o)16.6(d)12.8(i)]TJ /F12 1 Tf 7.1433 0 TD ()Tj /F11 1 Tf 0.5132 0 TD [(ed)-353(p)12.8(r)11.9(o)16.6(t)15.5(e)16.2(i)0(n)25.2(s)-364.1(a)13.3(l)17.3(on)26.5(e)16.2(,)]TJ /F12 1 Tf 7.4775 0 TD ()Tj /F11 1 Tf 0.5132 0 TD -0.015 Tc [(v)-15(e)-370.3(KEGG)-381.2(pathways)-379.1(we)-15(r)13.1(e)]TJ -15.6472 -1.2114 TD 0 Tc [(e)16.2(n)0(r)27.8(i)15.3(ch)28(ed)28.9(,)-313.1(m)18.3(a)13.3(i)15.3(nl)27.1(y)-311.8(a)13.3(m)18.3(i)15.3(no)-295.8(a)13.3(c)16.2(id)-300.1(b)16.6(i)15.3(o)16.6(s)11.9(y)16.4(n)0(t)25.4(h)17.8(es)28.1(i)15.3(s)11.9(,)-307.2(A)12.7(B)16.1(C)-310.8(t)15.5(r)11.9(a)13.3(n)15.8(s)11.9(p)12.8(o)16.6(r)17.9(te)25.7(r)11.9(s)17.9(,)-313.1(a)19.3(nd)]TJ T* [(c)16.2(a)13.3(t)15.5(i)15.3(on)26.5(i)15.3(c)-467.2(a)13.3(n)15.8(t)15.6(i)15.3(m)12.3(i)15.3(c)16.2(r)11.9(o)16.6(b)0(i)26(a)13.3(l)-460.2(p)12.8(ep)28.9(ti)24.9(d)12.7(e)-461.2(\()14.8(C)0(A)24.1(M)19.7(P)13(\))-468.6(r)17.9(e)0(s)28.1(i)15.3(s)11.9(t)15.5(a)13.3(n)15.8(ce)-451(\()]TJ /F20 1 Tf 0 0 0 rg 20.8749 0 TD -0.0149 Tc [(Fi)-14.9(g)13.2(u)0(re)-483.1(6C)]TJ /F11 1 Tf 0 0 0 1 k 4.237 0 TD (\),)Tj -25.1119 -1.2114 TD -0.0146 Tc [(whi)-14.6(l)12(e)-350.5(i)0.7(n)-350.8(676)-347.2(speci)]TJ /F12 1 Tf 7.1851 0 TD 0 Tc ()Tj /F11 1 Tf 0.5132 0 TD [(c)-335.9(a)13.3(c)16.2(e)0(t)25.7(y)16.4(la)24.6(t)15.6(e)16.2(d)-333.4(m)12.3(o)16.6(d)12.8(i)]TJ /F12 1 Tf 6.9464 0 TD ()Tj /F11 1 Tf 0.5192 0 TD [(e)16.2(d)-339.3(p)12.7(r)17.9(ot)26.2(e)16.2(i)15.3(ns)27.8(,)-337(w)14.6(e)16.2(r)11.9(e)-335.9(e)16.2(nr)27.7(i)15.3(c)0(h)28(e)0(d)]TJ -15.1638 -1.2114 TD -0.0149 Tc [(in)-285.5(six)-286.9(K)0.6(EGG)-279.6(p)-2.2(athways)-283.5(o)1.7(f)-284.9(w)-0.3(hic)-14.9(h)-273.4(me)-14.9(t)10.8(a)-1.6(bol)-14.9(i)11.7(c)-279.2(pathways,)-280.3(b)1.7(io)-14.9(s)13.6(y)-14.9(n)11.4(t)0.7(hesis)]TJ T* 0 Tc [(of)-498(ami)11.1(no)-498.7(ac)11.6(ids)-497.1(an)11.2(d)-506.4(p)0(y)11.2(rim)15.7(idi)13.6(ne)-499.1(met)14.2(ab)12(oli)13.4(sm)-494.9(are)-495.7(pre)11(do)11.5(min)13.7(an)11.2(t)]TJ 0 -1.2055 TD (\()Tj /F20 1 Tf 0 0 0 rg 0.3282 0 TD -0.014 Tc [(Fi)-14(g)14.1(u)0.9(re)-333(6)-14(D)]TJ /F11 1 Tf 0 0 0 1 k 4.1893 0 TD 0 Tc [(\))14.8(.)-319.1(F)12.7(u)12.8(r)11.9(t)15.6(h)17.8(e)0(r)-306.1(a)13.3(na)29.1(ly)27.7(s)11.9(i)15.3(s)-322.3(s)11.9(h)17.8(o)16.6(w)14.6(e)0(d)-311.2(t)15.6(h)11.8(a)19.3(t)-324.6(D)12.9(N)16.9(A)-321.5(b)16.6(i)0(n)25.2(d)12.8(i)15.3(n)15.8(g)-323.8(p)12.7(r)17.9(o)0(t)26.2(e)16.2(i)15.3(n)]TJ -4.5175 -1.2114 TD [(R)17.2(p)12.7(oB)-456.7(i)15.3(n)0(c)26(l)17.3(u)12.8(d)12.7(e)16.2(d)-470.6(2)13.5(8)-469.9(K)]TJ 7.1249 0 0 6.3326 387.0991 88.1008 Tm [(su)32.6(c)]TJ 9.5 0 0 9.5 400.592 89.6882 Tm -0.0151 Tc [(sites)-486.6(a)-1.8(nd)-485.7(6)-485(K)]TJ 7.1249 0 0 6.3326 454.3936 88.1008 Tm -0.0207 Tc (ace)Tj 9.5 0 0 9.5 467.6031 89.6882 Tm -0.0139 Tc [(sites,)-482.2(whil)-13.9(e)-469.8(f)-13.9(a)15.9(t)-13.9(t)11.2(y)-480.9(ac)-13.9(i)11.6(d)]TJ -16.9959 -1.2114 TD 0 Tc [(o)16.6(x)14.5(i)15.3(d)12.7(a)13.3(t)15.5(i)15.3(o)0(n)-212.2(c)16.2(om)28.9(p)12.7(l)17.3(ex)-214.1(s)17.9(u)12.8(b)16.6(u)12.7(ni)25.2(t)-223.2(a)13.3(l)17.3(p)12.7(h)17.8(a)-225.4(Y)16.3(f)0(c)26.7(X)-226.3(i)15.3(n)15.8(c)16.2(l)0(u)24(d)18.7(ed)-209.8(2)-225.2(K)]TJ 7.1249 0 0 6.3326 495.9495 76.5921 Tm [(su)32.6(c)]TJ 9.5 0 0 9.5 507.0613 78.1795 Tm -0.0146 Tc [(site)-14.6(s)-225.2(a)-1.3(nd)-240.6(17)]TJ -21.1494 -1.2114 TD 0 Tc (K)Tj 7.1249 0 0 6.3326 312.3212 65.0834 Tm [(ac)33.4(e)]TJ 9.5 0 0 9.5 325.1338 66.6708 Tm -0.014 Tc [(sites,)-440.5(s)3.9(u)-1.2(g)-14(g)12.8(ested)-442.9(t)1.5(ha)5.3(t)-440.1(t)1.5(here)-439.4(is)-437.7(a)-442.3(s)3.9(ig)-14(n)12.3(i)]TJ /F12 1 Tf 14.4656 0 TD 0 Tc ()Tj /F11 1 Tf 0.5192 0 TD [(c)16.2(a)13.3(n)15.9(t)-426.1(d)12.7(i)15.3(f)76.2(f)16.5(er)28.1(en)26(c)16.2(e)-425.4(in)-410.4(th)27.4(e)]TJ ET 0 0 0 0.7 k 45.354 717.505 m 549.921 717.505 l 549.694 717.222 l 45.581 717.222 l f* 549.921 717.505 m 549.921 313.682 l 549.694 313.965 l 549.694 717.222 l f* 549.921 313.682 m 45.354 313.682 l 45.581 313.965 l 549.694 313.965 l f* 45.354 313.682 m 45.354 717.505 l 45.581 717.222 l 45.581 313.965 l f* /EmbeddedDocument /MC13 BDC /Document /MC14 BDC q 398.912 699.427 -216.97 -186.652 re 181.941 699.427 m W n q 216.996033 0 0 186.665985 181.932007 512.772034 cm /Im10 Do Q Q q 141.732 486.659 311.809 -136.637 re W n q 311.855988 0 0 136.716019 141.702026 349.962006 cm /Im11 Do Q Q BT /F21 1 Tf 7 0 0 7 166.4723 693.95 Tm 0 0.003922 0 rg (A)Tj -2.699 -30.1433 TD (B)Tj ET EMC EMC BT /F1 1 Tf 6.9999 0 0 6.9999 50.6268 328.9322 Tm 0 0 0 1 k [(FIGURE)-299.4(4)]TJ /F7 1 Tf 5.0053 0 TD [(|)-429.9(Motif)-305.4(analysis)-299.4(of)-299.5(lysine)-300.3(succinylation)-308.4(sites.)]TJ /F1 1 Tf 18.6442 0 TD (\(A\))Tj /F7 1 Tf 1.5631 0 TD [(Sequence)-301.4(logos)-304.1(of)-299.5(motifs)-304.3(\()]TJ /F6 1 Tf 11.4279 0 TD (P)Tj /F7 1 Tf 0.6236 0 TD [(-value)-301(<0.000001\))-306.7(identi)]TJ /F8 1 Tf 10.3831 0 TD ()Tj /F7 1 Tf 0.4374 0 TD [(ed)-303(by)-298.3(MoMo)-305.4(software.)]TJ /F1 1 Tf 9.9781 0 TD (\(B\))Tj /F7 1 Tf 1.5874 0 TD [(Numbers)-304.9(of)-299.5(each)]TJ -59.6501 -1.3526 TD (identi)Tj /F8 1 Tf 2.2597 0 TD ()Tj /F7 1 Tf 0.4374 0 TD [(ed)-303(motifs.)]TJ ET 0 0 0 0.7 k 45.354 737.518 504.567 0.28345 re f BT 6.9999 0 0 6.9999 45.3543 744.2644 Tm [(Zeng)-305.3(et)-300.5(al.)]TJ 54.7663 0 TD [(Succinylation)-308.2(in)]TJ /F6 1 Tf 6.8924 0 TD [(V.)-305.2(alginolyticus)]TJ /F7 1 Tf 6.5765 0 TD (Virulence)Tj ET 45.354 42.803 504.567 0.22675 re f 45.354 42.803 504.567 0.22675 re f BT 6.9999 0 0 6.9999 45.3543 31.2945 Tm [(Frontiers)-306.1(in)-296.3(Cellular)-302.6(and)-306.5(Infection)-301.5(Microbiology)-499.5(|)-502.8(www.frontiersin.org)-23478.1(February)-305.6(2021)-505.2(|)-494.7(Volume)-307.7(10)-499.6(|)-494.7(Article)-305.6(626574)]TJ 35.7658 0 TD (7)Tj ET endstream endobj 156 0 obj<>stream Adobed         s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjzm!1AQa"q2#BRbr3$4CS%cs5DT &6E'dtU7()󄔤euFVfvGWgw8HXhx9IYiy*:JZjz ?N*U.3D)ŵ<f:ƿ _i*]v*UثWb]>TV!0Tс=-?)Gr> e\*ZJw ) NHHI 8)PaljSn]v*UثWb]v*UثWb]kP׷qs*A}d`?634m?^uh/`Z8dG 9\RbiܔO-A3A6kў2F"-r> Ԏ$ѳ"ճ<5Li[ v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]a>z}XCsyw\EoڪzQK;3*5qT[Ѥc{R}6ax劽!>b]v*UثWb_j׽/I3/B-v_ڝJap·98ddjtxb0~W`4 ?WhI,>%bf6)Xv*UثWb]v*UثWb]v*Ue~*xac-vV_?.T`>K8ATY`-/Ma0#};1Q\O e u|7}$瘮%Z)fg6e.?쿗 9/{8*1Ha%ʡs=/fǔ ,v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]y$tO5@j7}זwM 4r}S쯤[߳~rO6z徕iIhv!y7ǟ,U)ov*UثWb]v*+W"_g5 nӋ 2խQc{u6s-IGք_2l܀Lyb]v*UثWb]v*UثWb]$u,%~گ߳/aF?N?{Wު4r?՚(֘K6V2=W_#>/(R|ךêM 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ʒI|ayP[EE2'$ߕMk2y:,aĚ媷՝ =#drm~m6ѮxH*7Av*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb?Nԧ`J]\$;oLUC̞AҼum_Dу =銥>> endobj 202 0 obj<> endobj 203 0 obj<> endobj 204 0 obj<> endobj 205 0 obj<> endobj 206 0 obj<> endobj 207 0 obj<> endobj 208 0 obj<> endobj 209 0 obj<> endobj 210 0 obj<> endobj 211 0 obj<> endobj 212 0 obj<> endobj 213 0 obj<> endobj 214 0 obj<> endobj 215 0 obj<> endobj 216 0 obj<> endobj 217 0 obj<> endobj 218 0 obj<> endobj 219 0 obj<> endobj 220 0 obj<> endobj 221 0 obj<>/Border[0 0 0]/Type/Annot>> endobj 222 0 obj<>/Border[0 0 0]/Type/Annot>> endobj 223 0 obj<>/Border[0 0 0]/Type/Annot>> endobj 224 0 obj<>/Font<>/ProcSet[/PDF/Text/ImageC]/ExtGState<>>> endobj 225 0 obj<>stream /GS1 gs BT /F11 1 Tf 9.5 0 0 9.5 45.3543 710.4754 Tm 0 0 0 1 k 0.0204 Tc 0 Tw [(bac)-5.2(t)0.1(erial)-517.3(v)-5(irulenc)-5.2(e)0.8(,)-525.5(s)2.5(uch)-522.8(a)-2.1(s)-522.7(a)-2.1(ce)-5.2(tylat)-5.8(i)-0.1(on,)-525.5(s)-3.5(uc)-5.2(cin)-5.5(y)1(latio)-4.8(n)0.4(,)-525.5(a)3.9(n)-5.5(d)]TJ 0 -1.2114 TD 0.0116 Tc [(ph)-6.4(os)-6.3(phoryla)-4.9(t)3.3(ion)-515.6(\()]TJ 0 0 0 0.7 k 7.6446 0 TD 0.0127 Tc [(Whitmore)-514.2(and)-511.6(L)-5.3(amont,)-509.3(2)-3.6(0)2.4(1)-3.6(2)]TJ 0 0 0 1 k 12.729 0 TD 0 Tc (;)Tj 0 0 0 0.7 k 0.746 0 TD 0.0134 Tc [(Ren)-507.8(e)-0.3(t)-514.1(a)2.8(l.,)]TJ -21.1195 -1.2114 TD 0 Tc [(20)-8.8(17)]TJ 0 0 0 1 k 1.9335 0 TD (;)Tj 0 0 0 0.7 k 0.7221 0 TD [(G)-8.1(avi)-8.7(ar)-10.6(d)-506.4(e)0(t)-511.3(al.)-14(,)-504.1(2)0(0)-8.8(1)0(9)]TJ 0 0 0 1 k 8.5218 0 TD [(\).)-513.2(Vi)-9.8(rul)-11.8(enc)-11.5(e)-509(fac)-13.7(tor)-9.6(s)-507.3(\()0(V)-10.3(Fs\))-515.6(are)-513.6(t)-8.3(h)0(e)]TJ -11.1774 -1.2114 TD 0.0137 Tc [(basis)-517.5(o)0.5(f)-518.8(p)-3.4(at)-6.6(hog)-5.7(e)0(nic)-5.9(i)-0.8(ty)-518.9(of)]TJ /F15 1 Tf 11.3087 0 TD 0.0151 Tc [(V.)-513.8(alg)-4.5(i)-1.3(nolyticus)]TJ /F11 1 Tf 6.5406 0 TD 0.5291 Tc [(,s)511.2(oi)514.6(ti)514.6(so)509.9(fg)509.7(r)517.1(e)509.5(a)518.5(t)]TJ -17.8493 -1.2114 TD 0 Tc (signi)Tj /F12 1 Tf 1.8858 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(cance)-483.3(to)-481(study)-485.7(VFs.)-486.6(In)-480.7(this)-488.5(study,)-482.5(using)-482.8(online)-487.8(VFDB)]TJ -2.4169 -1.2114 TD [(software)-331.1(analysis,)-335.2(we)-327.3(detected)-332.3(a)-326.9(total)-333.5(of)-325(50)-331(\(7.45%)-331.3(of)-325(total)-333.5(K)]TJ 7.1249 0 0 6.3326 279.8362 651.4014 Tm (suc)Tj 9.5 0 0 9.5 45.3543 641.4802 Tm [(proteins\))-346.6(succinylated)-345.6(VFs)-340.6(in)]TJ /F15 1 Tf 12.2695 0 TD [(V.)-340.3(alginolyticus)]TJ /F11 1 Tf 6.1407 0 TD [(,)-343(and)-340(the)-344.4(protein-)]TJ -18.4102 -1.2114 TD [(protein)-397.1(interaction)-399.3(network)-399(among)-397(the)-398.1(50)-396.7(VFs)-394.3(proteins)-397.2(using)]TJ T* 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-23.256 -1.2114 TD [(et)-439.7(al.,)-440.5(2008)]TJ 0 0 0 1 k 4.6369 0 TD (;)Tj 0 0 0 0.7 k 0.6564 0 TD [(Goldberg)-443.5(et)-439.7(al.,)-440.5(2010)]TJ 0 0 0 1 k 8.7963 0 TD (;)Tj 0 0 0 0.7 k 0.6624 0 TD [(Kapitein)-441.2(and)-441.5(Mogk,)-439.8(2013)]TJ 0 0 0 1 k 10.6881 0 TD (;)Tj 0 0 0 0.7 k -25.4401 -1.2114 TD [(Erhardt,)-306.5(2016)]TJ 0 0 0 1 k 5.5201 0 TD (;)Tj 0 0 0 0.7 k 0.5192 0 TD [(Green)-300.1(and)-304.2(Mecsas,)-301.6(2016)]TJ 0 0 0 1 k 9.8645 0 TD (;)Tj 0 0 0 0.7 k 0.5192 0 TD [(Guo)-301(et)-302.5(al.,)-303.3(2017)]TJ 0 0 0 1 k 6.3914 0 TD (\).)Tj /F20 1 Tf 0 0 0 rg -21.5492 -1.2114 TD [(Figure)-289.8(9)]TJ /F11 1 Tf 0 0 0 1 k 3.7835 0 TD [(showed)-292.1(that)-289.9(S-ribosylhomocystein)-297.7(lyase)-289(\(LuxS\))-298.9(is)-289.1(a)]TJ -5.0486 -1.2055 TD [(key)-260.2(enzyme)-265(in)-261.2(quorum)-265.1(sensing)-260.6(and)-262.5(has)-261.4(two)-257.6(Ksuc)-265.9(sites.)-260.2(Previous)]TJ 0 -1.2114 TD [(studies)-327.4(have)-318.3(found)-325.4(that)-325.7(this)-321.4(enzyme)-324.7(plays)-322.3(an)-323(important)-324.4(role)-326(in)]TJ T* [(virulence)-255.9(\()]TJ 0 0 0 0.7 k 4.243 0 TD [(Coulthurst)-256.3(et)-248.8(al.,)-255.5(2004)]TJ 0 0 0 1 k 8.8262 0 TD [(\),)-250.6(and)-256.5(in)]TJ /F15 1 Tf 3.6224 0 TD [(A.)-248.9(hydrophila)]TJ /F11 1 Tf 5.6573 0 TD (research)Tj 5.1023 24.2108 TD [(it)-267.5(has)-267.3(been)-275.4(reported)-268.1(that)-272(LuxS)-268.8(exhibits)-270.7(cross-talk)-271.7(between)-270.8(lysine)]TJ 0 -1.2114 TD [(acetylation)-419.3(and)-423.6(succinylation,)-419(and)-423.6(Yao)-413.3(et)-421.8(al.)-419.8(research)-421.8(showed)]TJ T* [(that)-218.3(the)-225(succinylation)-225.3(of)-217.5(lysines)-223.4(on)-224.1(LuxS)-221(at)-221.8(the)-219(K23)-220(and)-226.7(K30)-220(sites)]TJ T* 0.024 Tc [(positively)-520.6(regulate)-526.8(the)-526.8(p)0.9(roduction)-521.1(of)-520.5(the)-520.8(q)2.9(u)-5(o)4.8(ru)-5(m)-518.7(s)0.1(ensing)]TJ T* 0.0099 Tc [(autoi)-4.6(n)1.9(duce)-3.8(r)-509.3(A)9.9(I)-8.5(-2,)-506.1(a)-0.7(n)-4.1(d)-508.5(that)-505.7(t)-4.4(h)9.9(e)-9.9(s)]TJ 13.8509 0 TD 0.0106 Tc [(e)-516.3(P)-0.3(TMs)-508.6(u)-0.5(l)4(t)2.3(imatel)4(y)-510.1(a)0(lter)-508.6(its)]TJ -13.8509 -1.2114 TD 0 Tc [(c)-7.7(o)0(m)-6.9(p)-11.1(et)-10.1(it)-10.9(iv)-10.1(e)-7.7(n)0(e)-9.8(s)0(s)-519.2(w)0(i)-11.9(t)0(h)]TJ /F15 1 Tf 9.2379 0 TD [(V)-8.3(.)-505(al)-12(g)-7.7(i)0(n)-10.6(o)-9.4(ly)-10.3(t)-8.3(i)0(c)-11.4(u)0(s)]TJ /F11 1 Tf 6.8926 0 TD (\()Tj 0 0 0 0.7 k 0.3521 0 TD [(Su)-8.6(n)-509.3(e)-7.7(t)-509.6(a)-10.6(l.)-9.4(,)-510.1(2)0(0)-14.7(1)0(9)]TJ 0 0 0 1 k 6.8747 0 TD (;)Tj 0 0 0 0.7 k 0.7281 0 TD 0.0076 Tc [(Ya)7.6(o)]TJ -24.0855 -1.2055 TD 0 Tc [(et)-338.3(al.,)-339.1(2019)]TJ 0 0 0 1 k 4.434 0 TD [(\).)-340.1(This)-343.6(study)-336.5(showed)-339.9(the)-338.4(succinylation)-344.6(of)-342.9(lysines)-336.8(on)]TJ -4.434 -1.2114 TD [(LuxS)-298.6(at)-293.4(the)-290.7(K29)-297.6(and)-298.3(K45)-291.6(sites.)-296(But)-295.8(whether)-295.9(they)-298.1(have)-294.4(the)-296.6(same)]TJ T* [(function)-293.2(as)-297.1(LuxS)-292.7(in)]TJ /F15 1 Tf 8.1399 0 TD [(A.)-290.6(hydrophila)]TJ /F11 1 Tf 5.7349 0 TD [(remains)-297.1(to)-296.1(be)-289.5(further)-297.3(studied)]TJ -13.8748 -1.2114 TD (in)Tj /F15 1 Tf 1.104 0 TD [(V.)-298.5(alginolyticus)]TJ /F11 1 Tf 6.0989 0 TD (.)Tj -5.9378 -1.2114 TD [(In)-421(Gram-negative)-434.4(bacteria,)-427.6(the)-427.9(Sec)-424.7(system)-429(can)-426.1(transport)-429.7(a)]TJ -1.2651 -1.2114 TD [(v)-7.5(a)0(r)-10.6(i)0(e)-10.3(t)-8.3(y)-508.8(o)-7.3(f)-508.7(p)0(r)-11.2(o)-7.3(t)-8.3(e)0(i)-10.3(n)0(s)-515.3(i)-8.5(nt)-10.4(o)-508.5(t)-8.3(he)-515.1(e)-7.7(x)0(t)-11.8(r)0(a)-10.6(c)-7.7(el)-8.3(l)-6.6(u)0(l)-11.8(a)0(r)-517.9(m)0(e)-13.3(d)0(i)-7.7(u)0(m)-16.7(,)-510.1(in)-10.6(c)-7.7(l)0(u)-11.8(d)0(i)-7.7(n)-8(g)]TJ 0 -1.2055 TD 0.0261 Tc [(tox)-7.2(i)-0.4(ns)-528.9(and)-528.1(e)-5.5(nzy)-5.2(m)2.6(e)-5.5(s)-528.9(\()]TJ 0 0 0 0.7 k 9.7512 0 TD 0.0266 Tc [(Chatzi)-531(et)-530.7(al.,)-531.2(2013)]TJ 0 0 0 1 k 8.3786 0 TD 0.0278 Tc [(\),)-530(which)-521.3(p)-1.2(lay)-522.7(a)-0.7(n)]TJ -18.1297 -1.2114 TD 0 Tc [(important)-437.8(role)-433.4(in)-434.3(bacterial)-437.3(virulence.)-437.7(In)-432.9(the)-439.9(Sec)-430.6(system)-440.9(SecA)]TJ T* [(plays)-334.2(a)-338.8(central)-335.4(role)-337.9(in)-338.8(directing)-342(Sec-dependent)-342.5(transport,)-337(while)]TJ T* [(SecE)-220.7(and)-220.7(SecY)-223.4(are)-221.2(membrane)-219.5(proteins)-224.1(that)-224.3(form)-217.2(a)-219.4(channel)-221.8(in)-225.4(the)]TJ T* [(memb)-8.2(rane)-509.7(whic)-7.8(h)-501.4(prov)-8(ides)-510.8(the)-505.5(core)-506.1(mo)-6.9(lecu)-9.3(lar)-506.6(machi)-8.7(nery)-506.6(to)]TJ T* 0.0311 Tc [(direct)-532.2(secret)4.9(ion)-531.9(\()]TJ 0 0 0 0.7 k 7.752 0 TD 0.0305 Tc [(Cr)-5.3(ane)-532.2(a)2(nd)-529.6(Randall,)-533.3(2017)]TJ 0 0 0 1 k 11.3027 0 TD 0.0324 Tc [(\).)-531.4(Anot)6.2(he)6.8(r)-534.6(S)5.1(ec)]TJ -19.0547 -1.2055 TD 0 Tc [(protein,)-316.4(YidC,)-316.9(is)-312.9(very)-315.1(important)-318.4(to)]TJ /F15 1 Tf 14.4477 0 TD [(E.)-317(coli)]TJ /F11 1 Tf 2.763 0 TD [(survival)-320.4(and)-310.2(deletion)]TJ -17.2107 -1.2114 TD 0.0139 Tc [(of)-512.7(YidC)-511.7(will)-511.9(interfere)-513(w)-1.3(ith)-511.4(t)-0.4(he)-513(insertion)-513.3(o)0.7(f)-518.6(S)4.5(ec)-5.7(-depe)-5.7(n)-0.1(dent)]TJ T* 0.0317 Tc [(memb)6.5(rane)-531(prot)5.5(eins,)-532.1(t)-0.5(hus)-535.3(a)3.2(f)60.1(f)0.4(ect)5.5(ing)-530.8(s)1.8(ecretion)-531.3(p)2.7(rocesses)-529.3(o)0.6(f)]TJ ET 0 0 0 0.7 k 45.354 460.063 m 549.921 460.063 l 549.694 459.836 l 45.581 459.836 l f* 549.921 460.063 m 549.921 66.557 l 549.694 66.784 l 549.694 459.836 l f* 549.921 66.557 m 45.354 66.557 l 45.581 66.784 l 549.694 66.784 l f* 45.354 66.557 m 45.354 460.063 l 45.581 459.836 l 45.581 66.784 l f* q 155.905 442.318 m 439.313 442.318 l 439.313 93.317 l 155.905 93.317 l W n q 283.407806 0 0 349.001495 155.905487 93.31649 cm /Im21 Do Q Q BT /F1 1 Tf 6.9999 0 0 6.9999 50.6268 72.2834 Tm 0 0 0 1 k 0 Tc [(FIGURE)-299.4(9)]TJ /F7 1 Tf 5.0053 0 TD [(|)-429.9(The)-302.2(PPI)-297.6(network)-306.9(of)-299.5(succinylated)-300.1(virulence)-309.4(factors)-295.2(in)]TJ /F6 1 Tf 22.9448 0 TD [(V.)-297.1(alginolyticus)]TJ /F7 1 Tf 6.2768 0 TD [(.)-305(The)-302.2(size)-295.5(of)-299.5(the)-304.1(circle)-298.6(represents)-305(the)-304.1(number)-301.9(of)-299.5(Ksuc)-305.3(sites.)]TJ ET 0 0 0 0.7 k 45.354 737.518 504.567 0.28345 re f BT 6.9999 0 0 6.9999 45.3543 744.2644 Tm [(Zeng)-305.3(et)-300.5(al.)]TJ 54.7663 0 TD [(Succinylation)-308.2(in)]TJ /F6 1 Tf 6.8924 0 TD [(V.)-305.2(alginolyticus)]TJ /F7 1 Tf 6.5765 0 TD (Virulence)Tj ET 45.354 42.803 504.567 0.22675 re f 45.354 42.803 504.567 0.22675 re f BT 6.9999 0 0 6.9999 45.3543 31.2945 Tm [(Frontiers)-306.1(in)-296.3(Cellular)-302.6(and)-306.5(Infection)-301.5(Microbiology)-499.5(|)-502.8(www.frontiersin.org)-23478.1(February)-305.6(2021)-505.2(|)-494.7(Volume)-307.7(10)-499.6(|)-494.7(Article)-305.6(626574)]TJ 35.4904 0 TD (10)Tj ET endstream endobj 226 0 obj<>stream Adobed      ""2  s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjzm!1AQa"q2#BRbr3$4CS%cs5DT &6E'dtU7()󄔤euFVfvGWgw8HXhx9IYiy*:JZjz ?N*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]1EZԯs$6;1Ab*KZuqZnMab,]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*Uv*8Xzbauy1$YD1k:y?Ydgչzc甜cAGe?F"=k9zeѓ+v:\+ȎP]}$p£[vTaG&4)v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWb]v*UثWU[ĶTT;7huyO6ЕzLYt[fvp!ɱӊH*$tŒd^g~lp\ @X>:`@y/ퟐtbdR#JU_:桯˽j>^lO/rQMrA8^[K!L5!^y}bH;MW6aJejY.0HWFu tB6ſ-{.`oN= 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[(er)-300.1(PXD023153.)]TJ /F1 1 Tf 11.9999 0 0 11.9999 306.1417 613.814 Tm [(AUTHOR)-302.7(CONTRIBUTIONS)]TJ /F11 1 Tf 9.5 0 0 9.5 306.1417 593.8014 Tm [(HP)-444.4(a)13.3(n)0(d)-442.9(W)0(L)-440.5(c)0(o)14.9(n)0(c)20.1(e)0(i)19.6(v)0(e)20.6(d)-452.7(t)15.5(h)0(e)-443.5(r)0(e)16.1(s)11.9(ea)17.5(r)11.9(c)0(h)-443.5(p)12.7(ro)16.6(je)21.5(ct)19.8(.)-450.4(F)0(Z)20.9(,)-456.3(YC)21.8(,)-450.4(a)0(n)17.2(d)-452.7(H)14(Z)]TJ 0 -1.2114 TD [(p)12.7(e)0(r)16.1(f)0(o)21.2(r)11.9(me)16.6(d)-267.7(t)0(h)21.4(e)-264.3(ex)18.7(pe)17(r)11.9(i)0(m)21.7(e)0(n)20.1(t)0(s)15.5(.)-265.4(W)13.2(L)0(,)-534(X)0(L)18.3(,)-271.4(a)13.3(n)0(d)-257.9(S)0(R)-254.8(p)0(e)17(r)11.9(fo)21.2(rm)18.3(ed)-257.5(th)21.4(e)-270.3(d)12.7(at)16.9(a)]TJ T* [(a)13.3(n)0(a)17.2(l)0(y)21.7(s)0(i)21.3(s)0(.)-420.6(H)0(P)21(,)-426.5(WL)19(,)-426.5(R)0(H)19.3(,)-426.5(SJ)14.2(M)13.7(,)-426.5(XL)-417.4(an)17.2(d)-428.9(J)11.7(J)-429.9(i)0(n)19.2(t)15.5(er)16.1(p)12.8(r)0(e)16.1(t)-426.1(th)21.4(e)-425.4(d)0(a)20.1(t)0(a)-418.7(a)0(n)17.2(d)]TJ 0 -1.2055 TD [(dis)10.1(cuss)11(ed)-502.2(the)-499.5(res)10.1(ults)15.7(.)-504.1(W)0(L)-506.1(wro)13.3(te)-499.4(the)-499.5(man)11.6(uscr)10.9(ipt.)-496.3(All)-495.9(auth)11.6(ors)]TJ 0 -1.2114 TD [(co)20.9(nt)19.5(r)11.9(i)0(b)20(u)0(t)22.3(e)0(d)-281.4(t)0(o)-278.1(t)0(h)15.4(e)-288.2(a)13.3(r)0(t)15.5(i)15.3(cl)15.5(e)-288.2(a)0(n)17.2(d)-285.6(ap)20.1(pr)18.7(ov)21.1(ed)-281.4(th)21.4(e)-294.2(s)11.9(u)12.7(b)0(m)17(i)0(t)18.9(t)15.5(ed)-281.4(ve)20.6(rs)17.8(io)20(n.)]TJ /F1 1 Tf 11.9999 0 0 11.9999 306.1417 507.5148 Tm (ACKNOWLEDGMENTS)Tj /F11 1 Tf 9.5 0 0 9.5 306.1417 487.5022 Tm [(This)-510.7(work)-506.3(wa)-7.9(s)-501.3(spon)-8.5(so)-7.3(red)-508.2(by)-504.1(gran)-8.2(ts)-503.7(fr)-7.4(om)-502.2(Shen)-13.3(zhen)-506.9(Scie)-9.5(nce)]TJ T* 0.0568 Tc [(and)-557.1(T)-3.5(echnology)-559.4(P)-1.8(roje)]TJ 10.4255 0 TD 0.0579 Tc [(ct)-559.1(\(JCYJ201908)5.8(13104207)5.8(152)-555.2(and)]TJ -10.4255 -1.2114 TD 0.0237 Tc [(JCYJ2)-4.6(0)1.4(1)-4.5(7)1.4(0)-4.6(8)1.4(1)-4.5(811)-4.6(16)-4.6(29)-4.6(778)-4.6(\),)-522.2(NSF)-5.3(C)-525.8(projects)-525.4(\()-3.3(Nos.)-528.1(32)-4.6(07)-4.5(301)-4.6(5,)]TJ 0 -1.2055 TD 0.0343 Tc [(31670129,)-529.5(and)-531.8(31802343\),)-535.4(S)1(outhern)-528.7(M)0.3(arine)-534.4(S)1(cience)-534.4(and)]TJ 0 -1.2114 TD 0 Tc [(Engineering)-500.2(Guangdong)-502.9(Laboratory)-494.5(\(Zhan)-503(jiang\))-497.9(\(No.)-497.6(ZJW-)]TJ T* [(2019-06\).)-358.5(The)-360.6(author)-353.7(SR)-362.2(thankfully)-359(acknowledges)-358.4(China)-361.9(Post-)]TJ T* [(doctoral)-381.1(Science)-385(Foundation)-377.8(for)-384.7(the)]TJ /F12 1 Tf 15.1698 0 TD ()Tj /F11 1 Tf 0.5311 0 TD [(nancial)-382.3(support)-383.2(to)-379.6(carry)]TJ -15.7009 -1.2114 TD [(out)-283.3(the)-284.7(Post-doctoral)-286.4(research)-284.5(work)-279.5(\(Grant)-288.8(No.)-279.6(2019M662214\).)]TJ T* [(We)-298.9(also)-304.9(thank)-301.9(Novogene)-304.5(Co.,)-299.8(Ltd.)-302.9(for)-301.1(the)-296.6(technical)-304(support.)]TJ /F1 1 Tf 11.9999 0 0 11.9999 306.1417 355.2377 Tm [(SUPPLEMENTARY)-309(MATERIAL)]TJ /F11 1 Tf 9.5 0 0 9.5 306.1417 335.2251 Tm [(T)17.2(h)11.8(e)-222.5(S)14.5(u)18.7(p)12.7(p)18.7(l)0(e)27.5(m)18.3(en)26(t)15.6(a)19.3(r)11.9(y)-222.3(M)13.7(a)19.3(t)15.6(e)16.2(r)11.9(i)15.3(a)19.3(l)-227.4(f)16.5(o)16.6(r)-220.8(t)15.6(h)11.8(i)15.3(s)-220.8(a)13.3(r)17.9(t)15.5(i)15.3(c)16.2(l)17.3(e)-222.5(ca)29.5(n)-222.9(b)16.6(e)-222.5(f)0(o)27.1(u)18.7(nd)-210.1(o)16.6(n)15.9(li)26.6(n)15.8(e)-222.5(a)19.3(t)15.6(:)]TJ T* [(https:/)-13.1(/www.fro)-9.8(ntier)-8.8(sin.o)-8.8(rg/arti)-11.4(cles/10.)-10(3389/fc)-9(imb.2)-10.7(020.6265)-9.6(74/)]TJ T* [(f)16.5(u)12.8(l)17.3(l)17.3(#)13.5(s)17.9(u)12.8(p)12.7(p)18.7(le)27.5(m)18.3(e)16.2(nt)31.4(a)13.3(r)11.9(y)16.4(-)19.8(m)12.3(a)13.3(t)15.5(e)16.2(r)17.9(i)15.3(a)13.3(l)]TJ /F1 1 Tf 6.9999 0 0 6.9999 306.1417 293.2157 Tm [(Supplementary)-309.1(Table)-297.6(1)-500.9(|)]TJ /F7 1 Tf 12.1001 0 TD [(The)-302.2(identi)]TJ /F8 1 Tf 4.1549 0 TD ()Tj /F7 1 Tf 0.4374 0 TD [(ed)-303(succinylated)-300.1(proteins)-301.7(and)-306.5(sites)-299.4(in)]TJ /F6 1 Tf -16.6923 -1.3607 TD [(V.)-297.1(alginolyticus)]TJ /F7 1 Tf 6.2768 0 TD (.)Tj /F1 1 Tf -6.2768 -2.7132 TD [(Supplementary)-260.5(Table)-257.1(2)-500.9(|)]TJ /F7 1 Tf 12.011 0 TD [(The)-253.6(identi)]TJ /F8 1 Tf 4.1063 0 TD ()Tj /F7 1 Tf 0.4374 0 TD [(ed)-262.5(succinylated)-259.6(peptides)-261.4(in)]TJ /F6 1 Tf 11.768 0 TD [(V.)-256.6(alginolyticus)]TJ /F7 1 Tf 6.2363 0 TD (.)Tj /F1 1 Tf 11.9999 0 0 11.9999 45.3543 230.6834 Tm (REFERENCES)Tj /F11 1 Tf 7.4999 0 0 7.4999 45.3543 212.6551 Tm [(Avison,)-282.9(M.)-280.3(B.,)-281.2(Bennett,)-279.1(P.)-278.3(M.,)-281.5(Howe,)-278.8(R.)-286.7(A.,)-275.4(and)-285.9(Walsh,)-278.3(T.)-279.2(R.)-279.2(\(2002\).)-279.2(Preliminary)]TJ 1.3304 -1.2624 TD [(analysis)-215(of)-214.4(the)-209.9(genetic)-214.6(basis)-206.7(for)-213.2(vancomycin)-218(resistance)-209.5(in)]TJ /F15 1 Tf 22.4734 0 TD [(Staphylococcus)-215.8(aureus)]TJ /F11 1 Tf -22.4734 -1.2699 TD [(strain)-256.7(Mu50.)]TJ /F15 1 Tf 5.367 0 TD [(J.)-252.7(Antimicrob.)-256.4(Chemother.)]TJ /F11 1 Tf 10.613 0 TD [(49,)-252.7(255)]TJ /F17 1 Tf 2.8649 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(260.)-249.9(doi:)-255.7(10.1093/jac/49.2.255)]TJ -20.6894 -1.2699 TD [(Avsever,)-239.8(M.)-235(L.)-233.7(\(2016\).)-241.4(First)-238.5(report)-232.6(of)]TJ /F15 1 Tf 14.7781 0 TD (trh)Tj /F11 1 Tf 1.4136 0 TD (positive)Tj /F15 1 Tf 3.3185 0 TD [(Vibrio)-234.5(alginolyticus)]TJ /F11 1 Tf 7.8993 0 TD [(isolates)-236.5(from)]TJ -26.0791 -1.2624 TD [(bivalve)-305.1(molluscs)-303.5(in)-302.6(Turkey.)]TJ /F15 1 Tf 11.3463 0 TD [(Rev.)-303.8(Med.)-299(Vet.)]TJ /F11 1 Tf 6.1532 0 TD [(167,)-302.8(65)]TJ /F17 1 Tf 2.9103 0 TD ()Tj /F11 1 Tf 0.514 0 TD (70.)Tj -22.2541 -1.2699 TD [(Cai,)-303.6(S.)-296.7(H.,)-300.7(Wu,)-297.2(Z.)-300.2(H.,)-300.7(Jian,)-300.7(J.)-299.1(C.,)-298.9(and)-301.1(Lu,)-303.3(Y.)-298.4(S.)-296.7(\(2007\).)-301.9(Cloning)-301.4(and)-301.1(expression)-299.8(of)]TJ 1.3304 -1.2624 TD [(gene)-429.2(encoding)-437.5(the)-429.1(thermostable)-429.9(direct)-428.2(hemolysin)-433.7(from)]TJ /F15 1 Tf 23.32 0 TD [(Vibrio)-431(alginolyticus)]TJ /F11 1 Tf -23.32 -1.2699 TD [(strain)-324.8(HY9901,)-313.4(the)-315.7(causative)-320.7(agent)-317.8(of)-320.2(vibriosis)-321(of)-312.6(crimson)-318.4(snapper)-322.7(\()]TJ /F15 1 Tf 27.6665 0 TD (Lutjanus)Tj -27.6665 -1.2624 TD 0.0368 Tc [(e)-6.6(r)0.2(yth)-5(o)-1.7(p)-5.8(t)-1.4(er)-7.4(us)]TJ /F11 1 Tf 5.3519 0 TD 0.0425 Tc (\).)Tj /F15 1 Tf 1.1792 0 TD 0.0411 Tc [(J.)-531.2(App)6.1(l)0.4(.)-538.7(M)1.4(icr)4.5(o)2.6(biol.)]TJ /F11 1 Tf 8.9425 0 TD 0.0426 Tc [(10)7.6(3,)-533.1(28)7.6(9)]TJ /F17 1 Tf 3.9081 0 TD 0 Tc ()Tj /F11 1 Tf 0.5443 0 TD 0.0358 Tc [(2)-6.8(9)-6.8(6)0.8(.)-539.9(d)-3.6(o)-8.5(i)1(:)-547.5(1)0.8(0)-6.8(.)-3.2(11)-6.8(11)-6.8(/)-5(j)-1.4(.1)-6.8(36)-6.8(5-)]TJ -19.9259 -1.2699 TD 0 Tc (2672.2006.03250.x)Tj -1.3304 -1.2699 TD [(Chatzi,)-226.3(K.)-219.6(E.,)-219(Sardis,)-226.3(M.)-219.8(F.,)-223.2(Karamanou,)-223.3(S.,)-222.3(and)-225.5(Economou,)-223.4(A.)-221.3(\(2013\).)-218.7(Breaking)-224(on)]TJ 1.3304 -1.2624 TD [(through)-407.2(to)-407.5(the)-406.4(other)-404.2(side:)-406.6(protein)-407.9(export)-408.3(through)-407.2(the)-406.4(bacterial)-412.7(Sec)-400.8(system.)]TJ /F15 1 Tf 0 -1.2699 TD [(Biochem.)-308(J.)]TJ /F11 1 Tf 4.7471 0 TD [(449,)-302.8(25)]TJ /F17 1 Tf 2.9178 0 TD ()Tj /F11 1 Tf 0.5065 0 TD [(37.)-305.6(doi:)-301.1(10.1042/BJ20121227)]TJ -9.5019 -1.2624 TD [(Chen,)-313.9(Y.)-313.5(Y.,)-322.3(Wu,)-312.3(F.)-312.7(L.,)-318(Pang,)-316.3(H.)-314.6(Y.,)-314.8(Tang,)-317.2(J.)-314.2(F.,)-321.5(Cai,)-311.2(S.,)-320.6(Cai,)-311.2(S.)-319.4(H.,)-315.8(et)-310(al.)-319.6(\(2019a\).)]TJ 1.3304 -1.2699 TD [(Su)-17.4(p)-9.1(e)-14.9(ro)-12.9(xi)-20.3(d)-9.1(e)-513.8(d)-9.1(i)-12.1(sm)-10.8(u)-9.1(t)-7.9(a)-9(s)-14(e)-513.8(B)-513.1(\()]TJ /F15 1 Tf 11.0817 0 TD [(so)-15.4(dB)]TJ /F11 1 Tf 1.9049 0 TD [(\))-12.3(,)-515.2(a)-9(n)-517.3(im)-16.5(p)-9.1(o)0(r)-12.9(t)-7.9(a)-9(n)-10.8(t)-514.4(v)-10.7(i)-12.1(r)0(u)-15.5(l)0(e)-14.3(n)-10.8(ce)-521.1(f)-11.4(a)-9(ct)-15.2(or)-526.9(of)]TJ /F15 1 Tf 15.6248 0 TD [(Vi)-16.4(b)-12.3(r)0(i)-16.1(o)]TJ -28.6114 -1.2624 TD (alginolyticus)Tj /F11 1 Tf 4.9059 0 TD [(,)-379.2(contributes)-383(to)-369.7(antioxidative)-387.4(stress)-373.4(and)-376.7(its)-381.7(potential)-380(application)]TJ 29.8662 21.5209 TD [(for)-288.8(live)-279(attenuated)-289.5(vaccine.)]TJ /F15 1 Tf 11.0364 0 TD [(Fish)-287(Shell)]TJ /F23 1 Tf 3.7645 0 TD ()Tj /F15 1 Tf 0.5216 0 TD [(sh)-283.3(Immunol.)]TJ /F11 1 Tf 5.3065 0 TD [(89,)-282.9(354)]TJ /F17 1 Tf 2.8952 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(360.)-280.1(doi:)-285.9(10.1016/)]TJ -24.0381 -1.2699 TD (j.fsi.2019.03.061)Tj -1.3304 -1.2699 TD [(Chen,)-253.4(Y.)-245.5(Y.,)-254.3(Wu,)-244.2(F.)-252.2(L.,)-250(Wang,)-250.4(Z.)-254.8(W.,)-251.4(Tang,)-249.2(J.)-253.7(F.,)-245.9(Cai,)-250.7(S.)-251.3(H.,)-247.8(and)-255.7(Jian,)-247.8(J.)-253.7(C.)-244.7(\(2019b\).)]TJ 1.3304 -1.2624 TD [(Construction)-237(and)-233(evaluation)-233(of)]TJ /F15 1 Tf 12.6087 0 TD [(Vibrio)-234.5(alginolyticus)]TJ /F24 1 Tf 7.8917 0 TD (D)Tj /F15 1 Tf 0.6047 0 TD (clpP)Tj /F11 1 Tf 1.8747 0 TD [(mutant,)-231.8(as)-234.6(a)-228.2(safe)-238.2(live)]TJ -22.9798 -1.2699 TD [(attenuated)-236.6(vibriosis)-222.8(vaccine.)]TJ /F15 1 Tf 11.3765 0 TD [(Fish)-234.1(Shell)]TJ /F23 1 Tf 3.7115 0 TD ()Tj /F15 1 Tf 0.514 0 TD [(sh)-230.4(Immunol.)]TJ /F11 1 Tf 5.1931 0 TD [(98,)-230(917)]TJ /F17 1 Tf 2.8422 0 TD 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[(93)-7.5(3.)]TJ -29.4278 -1.2699 TD 0 Tc [(doi:)-308.6(10.1021/acs.jproteome.8b00556)]TJ -1.3304 -1.2624 TD [(Goldberg,)-261.3(M.)-257.6(B.,)-258.5(Boyko,)-253(S.)-258.9(A.,)-260.3(Butterton,)-260.7(J.)-261.3(R.,)-257.7(Stoebner,)-258.6(J.)-253.7(A.,)-260.3(and)-263.3(Calderwood,)-256(S.)]TJ 1.3304 -1.2699 TD [(B.)-227(\(2010\).)-226.3(Characterization)-224.6(of)-221.9(a)]TJ /F15 1 Tf 12.6087 0 TD [(Vibrio)-226.9(cholerae)]TJ /F11 1 Tf 6.2061 0 TD [(virulence)-221.7(factor)-229.9(homologous)-220.3(to)]TJ -18.8147 -1.2624 TD 0.018 Tc [(t)-5(h)4.7(e)-526(f)-0.9(amily)-521.8(o)4(f)-522.5(T)-3(onB-de)-4.4(pe)-4.4(nde)-4.4(n)-0.4(t)-519.1(p)1.3(rot)-5(e)3.1(ins.)]TJ /F15 1 Tf 18.0513 0 TD 0.0178 Tc [(Mol.)-524.2(Microbio)-5.5(l.)]TJ /F11 1 Tf 7.1434 0 TD 0.0199 Tc [(6,)-518(2)7.6(407)]TJ /F17 1 Tf 3.2429 0 TD 0 Tc ()Tj /F11 1 Tf 0.5291 0 TD 0.0199 Tc [(24)7.6(18.)]TJ -28.9667 -1.2699 TD 0 Tc [(doi:)-308.6(10.1111/j.1365-2958.1992.tb01415.x)]TJ -1.3304 -1.2699 TD [(Green,)-451.3(E.)-452.1(R.,)-446.7(and)-452.2(Mecsas,)-452.8(J.)-450.2(\(2016\).)-453.1(Bacterial)-450.6(secretion)-450(systems:)-451.3(an)-450.7(overview.)]TJ /F15 1 Tf 1.3304 -1.2624 TD [(Microbiol.)-309.5(Spectr.)]TJ /F11 1 Tf 7.2492 0 TD [(4,)-300.8(213)]TJ /F17 1 Tf 2.434 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(239.)-295.2(doi:)-308.6(10.1128/microbiolspec.VMBF-0012-2015)]TJ -11.5277 -1.2699 TD [(Guo,)-268.8(M.)-265.2(L.,)-272.7(Huang,)-271(Z.)-270(W.,)-266.6(and)-270.8(Yang,)-268.4(J.)-268.8(\(2017\).)-271.7(Is)-263(there)-269(any)-272.4(crosstalk)-270.9(between)-269.6(the)]TJ 1.3304 -1.2624 TD [(chemotaxis)-339.4(and)-331.3(virulence)-342.6(induction)-335.3(signaling)-339(in)]TJ /F15 1 Tf 19.8957 0 TD [(Agrobacterium)-338.7(tumefaciens)]TJ /F11 1 Tf 10.9003 0 TD (?)Tj /F15 1 Tf -30.796 -1.2699 TD [(Biotechnol.)-304.9(Adv.)]TJ /F11 1 Tf 7.4999 0 0 7.4999 106.2425 350.929 Tm [(35,)-305.6(505)]TJ /F17 1 Tf 2.9178 0 TD ()Tj /F11 1 Tf 0.5065 0 TD [(511.)-302.8(doi:)-301.1(10.1016/j.biotechadv.2017.03.008)]TJ -11.5428 -1.2624 TD [(He,)-299.2(D.)-300.3(L.,)-295.4(Wang,)-295.8(Q.,)-299(Li,)-298.7(M.,)-304.2(Damaris,)-294.6(R.)-301.9(N.,)-299(Yi,)-295.4(X.)-301.1(L.,)-295.4(Cheng,)-301.9(Z.)-292.6(Y.,)-299.6(et)-302.5(al.)-296.9(\(2016\).)]TJ 1.3304 -1.2699 TD [(Global)-408.9(proteome)-410.6(analyses)-406.7(of)-410.9(lysine)-402(acetylation)-413(and)-406.9(succinylation)-411.9(reveal)-410.5(the)]TJ T* [(widespread)-275.8(involvement)-269.3(of)-267.3(both)-260.9(modi)]TJ /F12 1 Tf 15.2392 0 TD ()Tj /F11 1 Tf 0.5291 0 TD [(cation)-272.8(in)-264.8(metabolism)-268(in)-264.8(the)-270.4(embryo)-260.9(of)]TJ -15.7684 -1.2624 TD 0.0208 Tc [(g)-5(e)-1.6(rmin)-5.1(atin)-5.1(g)-519(r)-0.7(i)-6.4(c)-1.6(e)-515.7(s)-0.7(eed.)]TJ /F15 1 Tf 10.2426 0 TD 0.0217 Tc [(J.)-520.3(Prot)6.2(e)-6.6(o)5.9(m)-6.1(e)-520.6(Res.)]TJ /F11 1 Tf 7.6801 0 TD 0.0199 Tc [(15)-7.6(,)-518(8)0(7)-7.6(9)]TJ /F17 1 Tf 3.2656 0 TD 0 Tc ()Tj /F11 1 Tf 0.5291 0 TD 0.0208 Tc [(89)-6.7(0.)-517.1(doi:)-524.7(10.10)-6.7(21/acs.)]TJ -21.7174 -1.2699 TD 0 Tc (jproteome.5b00805)Tj -1.3304 -1.2624 TD (Herna)Tj /F16 1 Tf 2.4718 0.0378 TD ()Tj /F11 1 Tf 0.0454 -0.0378 TD [(ndez-Robles,)-378.9(M.)-371(F.,)-374.4(lvarez-Contreras,)-377(A.)-372.5(K.,)-372(Jua)]TJ /F16 1 Tf 19.5102 0.0378 TD ()Tj /F11 1 Tf 0.0529 -0.0378 TD (rez-Garc)Tj /F25 1 Tf 3.4772 0 TD ()Tj /F16 1 Tf 0.2872 0.0378 TD ()Tj /F11 1 Tf -0.0302 -0.0378 TD [(a,)-373(P.,)-370.2(Natividad-)]TJ -24.4841 -1.2699 TD [(Bonifacio,)-310.2(I.,)-304.4(Curiel-Quesada,)-317(E.,)-302.2(Va)]TJ /F16 1 Tf 14.5589 0.0454 TD ()Tj /F11 1 Tf 0.0529 -0.0454 TD [(zquez-Salinas,)-311.7(C.,)-306.4(et)-302.5(al.)-304.5(\(2016\).)-309.5(Virulence)]TJ -14.6118 -1.2624 TD 0.0154 Tc [(fac)-7(t)-0.1(or)-6.1(s)-512.6(a)-1.2(nd)-522.9(anti)-4.3(mi)-4.3(cr)-6.1(obi)-4.3(al)-520.8(re)-7(si)-4.3(stanc)-7(e)-513.5(i)-4.3(n)-517(envi)-4.3(ro)-6.2(nm)-4.1(ent)-7.6(a)-1.2(l)-513.2(s)-6.1(trai)-4.3(ns)-520.1(of)]TJ /F15 1 Tf 28.566 0 TD 0.0184 Tc [(Vib)6.1(r)-3.1(io)]TJ -28.566 -1.2699 TD 0 Tc (alginolyticus)Tj /F11 1 Tf 4.9059 0 TD (.)Tj /F15 1 Tf 0.5216 0 TD [(Int.)-298.2(Microbiol.)]TJ /F11 1 Tf 6.0398 0 TD [(19,)-298(191)]TJ /F17 1 Tf 2.9103 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(198.)-302.8(doi:)-301.1(10.2436/20.1501.01.277)]TJ -16.2219 -1.2699 TD [(Horii,)-215.6(T.,)-219.9(Morita,)-223.9(M.,)-213.5(Muramatsu,)-228.2(H.,)-217.5(Monji,)-218.4(A.,)-215(Miyagishima,)-221.3(D.,)-218.3(Kanno,)-226.5(T.,)-212.4(et)-219.3(al.)]TJ 1.3304 -1.2624 TD [(\(2005\).)-279.2(Antibiotic)-273.1(resistance)-277.5(in)]TJ /F15 1 Tf 12.6313 0 TD [(Aeromonas)-277.2(hydrophila)]TJ /F11 1 Tf 9.3053 0 TD (and)Tj /F15 1 Tf 1.7688 0 TD [(vibrio)-274.5(alginolyticus)]TJ /F11 1 Tf -23.7055 -1.2699 TD [(i)-12.1(sol)-12.3(a)-9(ted)-523.3(fr)-10.2(om)-517.3(a)-507.9(w)0(o)-9.8(u)-9.1(nd)-518.9(inf)-19.2(ect)-15(io)-11(n:)-518.5(a)-507.9(c)0(a)-16.3(s)0(e)-512.6(r)0(e)-13.7(por)-14.4(t)-7.9(.)]TJ /F15 1 Tf 20.9842 0 TD [(J.)-517.3(T)-11.7(r)0(a)-8.7(u)0(m)-10.8(a)]TJ /F11 1 Tf 4.8454 0 TD [(58)-17.1(,)-507.7(1)0(9)-9.6(6)]TJ /F17 1 Tf 3.1522 0 TD ()Tj /F11 1 Tf 0.5216 0 TD [(20)-9.6(0.)]TJ -29.5034 -1.2624 TD [(doi:)-308.6(10.1097/01.ta.0000066381.33339.c0)]TJ -1.3304 -1.2699 TD [(Huang,)-218.1(L.)-211(X.,)-219.2(Xu,)-212(W.,)-213.6(Su,)-215.1(Y.)-215.3(Q.,)-215.9(Zhao,)-215.5(L.)-211(M.,)-213.5(and)-217.9(Yan,)-212.4(Q.)-214.7(P.)-217.8(\(2018\).)-211.2(Regulatory)-224(role)]TJ 1.3304 -1.2624 TD [(o)-14(f)-525.4(t)-7.9(h)-13.3(e)-521.3(R)-13.4(st)-21.9(B-)-23(Rs)-19.8(t)-7.9(A)-522.5(s)-14(y)-10.7(s)-14(t)-7.9(e)-14.9(m)-518.4(i)-12.1(n)-524.8(a)-9(d)-16.7(he)-20.6(s)-14(i)0(o)-18.6(n)-10.8(,)-522.8(b)-14(i)-12.1(o)]TJ /F12 1 Tf 18.2402 0 TD ()Tj /F11 1 Tf 0.5443 0 TD [(lm)-533(p)-9.1(r)-14(o)-14(d)-9.1(u)-9.1(c)-14.9(t)-7.9(i)-12.1(o)-14(n)-10.8(,)-522.8(m)-11.9(ot)-21.9(i)-12.1(l)0(i)-19.1(t)-7.9(y)-18.2(,)-515.2(a)-16.6(n)-10.8(d)]TJ -18.7845 -1.2699 TD (hemolysis.)Tj /F15 1 Tf 4.4826 0 TD (MicrobiologyOpen)Tj /F11 1 Tf 7.5365 0 TD [(7,)-300.8(e00599.)-304.5(doi:)-301.1(10.1002/mbo3.599)]TJ -13.3495 -1.2699 TD 0.0199 Tc [(Ji)-7.3(n,)-525.6(W.)-518(B.,)-518(a)-4.2(nd)-525.9(Wu,)-525.6(F)-4.4(.)-518(L)-0.9(.)-525.6(\()0.1(2016)]TJ 14.6572 0 TD 0.0205 Tc [(\).)-517.4(Proteome-w)-5.5(ide)-516(i)-6.7(denti)]TJ /F12 1 Tf 10.4392 0 TD 0 Tc ()Tj /F11 1 Tf 0.5518 0 TD 0.0221 Tc [(cation)-517.9(of)-518.4(lysine)]TJ -24.3178 -1.2624 TD 0 Tc [(succinylation)-434.5(in)-423.6(the)-421.6(proteins)-429.4(of)-426(Tomato)-425.6(\()]TJ /F15 1 Tf 17.4465 0 TD [(Solanum)-426.2(lycopersicum)]TJ /F11 1 Tf 8.9727 0 TD (\).)Tj /F15 1 Tf 0.9902 0 TD [(PloS)-422.2(One)]TJ /F11 1 Tf -27.4095 -1.2699 TD [(11,)-305.6(e0147586.)-299(doi:)-301.1(10.1371/journal.pone.0147586)]TJ -1.3304 -1.2624 TD [(Kapitein,)-424.1(N.,)-427.5(and)-422(Mogk,)-420.7(A.)-425.4(\(2013\).)-422.8(Deadly)-417.7(syringes:)-427.2(type)-428.1(VI)-421.3(secretion)-419.8(system)]TJ 1.3304 -1.2699 TD 0.0272 Tc [(activities)-523.5(in)-527.9(patho)5.6(g)1.4(enicity)-527.7(a)3.1(nd)]TJ 14.0298 0 TD 0.0281 Tc [(int)5.1(e)-1.9(rb)6.5(ac)5.7(te)5.7(rial)-523.2(compet)5.1(it)5.1(ion.)]TJ /F15 1 Tf 12.0568 0 TD 0.0243 Tc [(Curr.)-532.9(O)-4.4(p)4.4(i)-8.1(n.)]TJ -26.0866 -1.2624 TD 0 Tc (Microbiol.)Tj /F11 1 Tf 4.3465 0 TD [(16,)-298(52)]TJ /F17 1 Tf 2.434 0 TD ()Tj /F11 1 Tf 0.5065 0 TD [(58.)-305.6(doi:)-301.1(10.1016/j.mib.2012.11.009)]TJ -8.6174 -1.2699 TD 0.0162 Tc [(Ko)-5.4(mine)-6.2(-Ab)-5.4(e)1.3(,)-521.7(A)0.2(.,)-521.7(Nagano)-5.4(-Shoj)-5.9(i)-3.5(,)-514.2(M)-5.9(.,)-521.7(Kub)-5.4(o)2.2(,)-521.7(S)0.4(.,)-521.7(K)-5.7(a)-0.4(wasa)-7.9(ki,)-521.7(H)-2.4(.,)-521.7(Yos)-5.3(h)-4.6(ida)-7.9(,)-514.2(M)-5.9(.)-0.1(,)]TJ 1.3304 -1.2699 TD 0 Tc [(Nishiyama,)-367.2(M.,)-364.7(et)-363(al.)-357.4(\(2017\).)-362.4(Ef)59.3(fect)-358.9(of)-365.5(lysine)-364.2(succinylation)-366.5(on)-357.4(the)-361.1(regulation)]TJ 0 -1.2624 TD 0.014 Tc [(of)-511.4(2)-5.9(-)5.2(oxoglut)6.1(a)-2.5(rat)6.1(e)-514.9(dehydro)14(g)-10.7(enase)-507.3(i)-5.7(nh)]TJ 15.8289 0 TD 0.0142 Tc [(ibitor,)-508.6(OdhI,)-508.6(i)2.1(nvolved)-516.5(i)2.1(n)-510.6(g)3.5(lutamate)]TJ -15.8289 -1.2699 TD 0 Tc [(production)-364.6(in)]TJ /F15 1 Tf 5.9717 0 TD [(Corynebacterium)-360(glutamicum)]TJ /F11 1 Tf 11.883 0 TD (.)Tj /F15 1 Tf 0.5821 0 TD [(Biosci.)-355.4(Biotechnol.)-365.4(Biochem.)]TJ /F11 1 Tf 11.5579 0 TD (81,)Tj -29.9947 -1.2624 TD (2130)Tj /F17 1 Tf 1.92 0 TD ()Tj /F11 1 Tf 0.5065 0 TD [(2138.)-307.6(doi:)-301.1(10.1080/09168451.2017.1372182)]TJ -3.7569 -1.2699 TD [(Kosono,)-339.4(S.,)-343.3(Tamura,)-339.7(M.,)-342(Suzuki,)-336.7(S.,)-343.3(Kawamura,)-337.8(Y.,)-345(Yoshida,)-339.6(A.,)-335.9(Nishiyama,)-344.5(M.,)]TJ 1.3304 -1.2624 TD [(et)-287.4(al.)-281.8(\(2015\).)-286.8(Changes)-285.8(in)-280(the)-285.5(acetylome)-286.6(and)-285.9(succinylome)-285.4(of)]TJ /F15 1 Tf 23.9927 0 TD [(Bacillus)-285.5(subtilis)]TJ /F11 1 Tf 6.3799 0 TD (in)Tj 4.3994 84.8666 TD [(response)-294.5(to)-294.1(carbon)-288.3(source.)]TJ /F15 1 Tf 11.0137 0 TD [(PloS)-293.7(One)]TJ /F11 1 Tf 3.9232 0 TD [(10,)-290.5(e0131169.)-299(doi:)-285.9(10.1371/journal.pone.)]TJ -14.9369 -1.2699 TD (0131169)Tj -1.3304 -1.2624 TD [(Li,)-245.8(X.)-248.2(L.,)-250(Hu,)-248.1(X.,)-249.4(Wan,)-247.3(Y.)-245.5(J.,)-254.9(Xie,)-244.9(G.)-245.7(Z.,)-248.5(Li,)-253.4(X.)-248.2(Z.,)-248.5(Chen,)-245.9(D.,)-248.6(et)-249.6(al.)-244(\(2014\).)-249(Systematic)]TJ 1.3304 -1.2699 TD (identi)Tj /F12 1 Tf 2.3055 0 TD ()Tj /F11 1 Tf 0.5291 0 TD [(cation)-250.1(of)-244.6(the)-255.3(lysine)-243.3(succinylation)-253.1(in)-249.7(the)-247.7(protozoan)-251.4(parasite)]TJ /F15 1 Tf 23.5241 0 TD (Toxoplasma)Tj -26.3588 -1.2624 TD (gondii)Tj /F11 1 Tf 2.4643 0 TD (.)Tj /F15 1 Tf 0.514 0 TD [(J.)-298.1(Proteome)-311.5(Res.)]TJ /F11 1 Tf 6.7276 0 TD [(13,)-298(6087)]TJ /F17 1 Tf 3.3865 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(6095.)-300(doi:)-301.1(10.1021/pr500992r)]TJ -14.9369 -1.2699 TD [(Nadine,)-399.1(P.,)-392.9(Serge,)-396.4(G.,)-390.5(and)-391.8(Yang,)-396.9(X.)-391.8(J.)-397.3(\(2017\).)-392.6(Assays)-395(for)-394.6(acetylation)-397.9(and)-391.8(other)]TJ 1.3304 -1.2699 TD [(acylations)-305.9(of)-305.1(lysine)-303.8(residues.)]TJ /F15 1 Tf 11.7091 0 TD [(Curr.)-305.9(Protoc.)-303.4(Protein)-300.4(Sci.)]TJ /F11 1 Tf 7.4999 0 0 7.4999 481.1526 654.9164 Tm [(87,)-305.6(1)]TJ /F17 1 Tf 1.9578 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(18.)-305.6(doi:)-301.1(10.1002/)]TJ -24.4765 -1.2624 TD (cpps.26)Tj -1.3304 -1.2699 TD [(Pan,)-313.2(J.)-321.7(Y.,)-314.8(Chen,)-313.9(R.,)-318.2(Li,)-313.8(C.)-320.3(C.,)-314(Li,)-313.8(W.)-318.3(Y.,)-314.8(and)-316.2(Ye,)-320.9(Z.)-315.3(C.)-312.8(\(2015\).)-317(Global)-318.2(analysis)-313.3(of)]TJ 1.3304 -1.2624 TD [(protein)-226.5(lysine)-228.2(succinylation)-230.4(pro)]TJ /F12 1 Tf 12.5331 0 TD ()Tj /F11 1 Tf 0.5291 0 TD [(les)-224.8(and)-225.5(their)-228.4(overlap)-229.8(with)-218(lysine)-228.2(acetylation)-231.6(in)]TJ -13.0622 -1.2699 TD [(the)-255.3(marine)-261.7(bacterium)]TJ /F15 1 Tf 8.882 0 TD [(Vibrio)-257.2(parahemolyticus)]TJ /F11 1 Tf 9.2373 0 TD (.)Tj /F15 1 Tf 0.4762 0 TD [(J.)-252.7(Proteome)-258.6(Res.)]TJ /F11 1 Tf 6.584 0 TD [(14,)-252.7(4309)]TJ /F17 1 Tf 3.3487 0 TD ()Tj /F11 1 Tf 0.514 0 TD (4318.)Tj -29.0423 -1.2624 TD [(doi:)-301.1(10.1021/acs.jproteome.5b00485)]TJ -1.3304 -1.2699 TD [(Pang,)-255.8(H.)-261.7(Y.,)-254.3(Li,)-260.9(W.)-257.8(X.,)-257(Zhang,)-260.7(W.)-257.8(J.,)-254.9(Zhou,)-261(S.)-258.9(H.,)-255.3(Hoare,)-260.6(R.,)-257.7(Monaghan,)-259.6(S.)-258.9(J.,)-254.9(et)-257.1(al.)]TJ 1.3304 -1.2699 TD [(\(2020\).)-317(Acetylome)-308.7(pro)]TJ /F12 1 Tf 9.0861 0 TD ()Tj /F11 1 Tf 0.5367 0 TD [(ling)-305.2(of)]TJ /F15 1 Tf 2.9178 0 TD [(Vibrio)-310.1(alginolyticus)]TJ /F11 1 Tf 8.0429 0 TD [(reveals)-311.1(its)-313.7(role)-306.9(in)-310.2(bacterial)]TJ -20.5836 -1.2624 TD (virulence.)Tj /F15 1 Tf 4.1651 0 TD [(J.)-305.6(Proteomics)]TJ /F11 1 Tf 5.4577 0 TD [(211,)-302.8(103543.)-302(doi:)-301.1(10.1016/j.jprot.2019.103543)]TJ -10.9532 -1.2699 TD [(Ren,)-312.4(J.,)-315.4(Sang,)-319.6(Y.,)-314.8(Lu,)-318.4(J.,)-315.4(and)-316.2(Yao,)-313.9(Y.)-313.5(F.)-312.7(\(2017\).)-317(Protein)-313(acetylation)-322.3(and)-316.2(its)-313.7(role)-314.4(in)]TJ 1.3304 -1.2624 TD [(bacterial)-473.1(virulence.)]TJ /F15 1 Tf 8.1866 0 TD [(Trends)-466.4(Microbiol.)]TJ /F11 1 Tf 7.6952 0 TD [(25,)-464.3(768)]TJ /F17 1 Tf 3.0766 0 TD ()Tj /F11 1 Tf 0.514 0 TD [(779.)-461.5(doi:)-474.9(10.1016/j.tim.2017.)]TJ -19.4724 -1.2699 TD (04.001)Tj -1.3304 -1.2624 TD 0.0391 Tc [(Rib)-5.2(e)1.6(t,)-544.2(D.,)-536.6(a)-0.1(nd)-544.5(Cos)-5.1(s)2.5(a)-7.7(r)2.5(t,)-544.2(P.)-536.6(\(2010\).)-544.2(P)3.9(a)-7.7(t)0.9(ho)-5.2(gen-mediate)-6(d)-537(p)-7.8(o)2.4(st)-6.6(tra)-7.7(n)5.6(s)-5.1(l)1.8(at)-6.6(io)-5.2(nal)]TJ 1.3304 -1.2699 TD 0 Tc (modi)Tj /F12 1 Tf 2.0939 0 TD ()Tj /F11 1 Tf 0.5367 0 TD [(cations:)-303.1(a)-303.8(re-emerging)]TJ /F12 1 Tf 9.2524 0 TD ()Tj /F11 1 Tf 0.5291 0 TD (eld.)Tj /F15 1 Tf 1.7159 0 TD (Cell)Tj /F11 1 Tf 1.7991 0 TD [(143,)-302.8(694)]TJ /F17 1 Tf 3.4016 0 TD ()Tj /F11 1 Tf 0.5065 0 TD [(702.)-310.4(doi:)-301.1(10.1016/j.cell.2010.)]TJ -19.8352 -1.2699 TD (11.019)Tj -1.3304 -1.2624 TD [(Samuelson,)-357.2(J.)-352(C.,)-351.8(Chen,)-351.7(M.)-348.4(Y.,)-352.6(Jiang,)-356.8(F.)-350.5(L.,)-348.3(Mller,)-359.8(I.,)-349.7(and)-354(Dalbey,)-348.2(R.)-354.8(E.)-353.9(\(2000\).)]TJ 1.3304 -1.2699 TD [(YidC)-297.4(mediates)-290.3(membrane)-297.9(protein)-294.5(insertion)-292(in)-295.1(bacteria.)]TJ /F15 1 Tf 22.5489 0 TD (Nature)Tj /F11 1 Tf 3.0766 0 TD [(406,)-287.7(637)]TJ /F17 1 Tf 3.3789 0 TD ()Tj /F11 1 Tf 0.514 0 TD (641.)Tj -29.5185 -1.2624 TD [(doi:)-301.1(10.1038/35020586)]TJ -1.3304 -1.2699 TD [(Santhakumari,)-231.2(S.,)-222.3(Nilofernisha,)-233.7(N.)-222.2(M.,)-228.6(Ponraj,)-227.2(J.)-223.5(G.,)-224.2(Pandian,)-225.7(S.)-228.7(K.,)-220.8(and)-225.5(Ravi,)-227.8(A.)-228.9(V.)]TJ 1.3304 -1.2624 TD 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First Succinylome Profiling of Vibrio alginolyticus Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence Fuyuan Zeng Huanying Pang Ying Chen Hongwei Zheng Wanxin Li Srinivasan Ramanathan Rowena Hoare Sean J. Monaghan Xiangmin Lin Jichang Jian Recent studies have shown that a key strategy of many pathogens is to use post-translational modification (PTMs) to modulate host factors critical for infection Vibrio alginolyticus lysine succinylation acetylation crosstalk virulence factors uuid:8ef69e8d-1730-48a4-bef4-4edf457f326c uuid:2d05245a-2bac-47c5-ab43-b424a24ecc3a endstream endobj xref 488 1 0002524621 00000 n 611 2 0002524794 00000 n 0002524851 00000 n 635 2 0002525231 00000 n 0002525856 00000 n trailer <<0592CB32E744FA448E993AAD49E9A8D9>]/Prev 116 >> startxref 2530860 %%EOF